Expression profiling and validation of potential reference genes during Paralichthys olivaceus embryogenesis.

Marine Biotechnology
Qiwang ZhongXun Lan

Abstract

Differential expression of genes is crucial to embryogenesis. The analysis of gene expression requires appropriate references that should be minimally regulated during the embryonic development. To select the most stable genes for gene normalization, the expression profiles of eight commonly used reference genes (ACTB, GAPDH, rpL17, alpha-Tub, EF1-alpha, UbcE, B2M, and 18S rRNA) were examined during Japanese flounder (Paralichthys olivaceus) embryonic development using quantitative real-time polymerase chain reaction. It was found that all seven mRNA genes appeared to be developmentally regulated and exhibited significant variation of expression. However, further analyses revealed the stage-specific expression stability. Hence when normalization using these mRNA genes, the differential and stage-related expression should be considered. 18S rRNA gene, on the other hand, showed the most stable expression and could be recommended as a suitable reference gene during all embryonic developmental stages in P. olivaceus. In summary, our results provided not only the appropriate reference gene for embryonic development research in P. olivaceus, but also possible guidance to reference gene selection for embryonic gene expression analyses...Continue Reading

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Citations

Sep 15, 2009·Journal of Biomedicine & Biotechnology·Airong Jia, Xiao-Hua Zhang
Jul 2, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jing WangQuanqi Zhang
Apr 29, 2015·International Journal of Molecular Sciences·Lin FanXubo Wang
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May 15, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xiaosu ZhouQuanqi Zhang
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Sep 23, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Duk-Young Kang, Hyo-Chan Kim
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Jul 29, 2011·The Journal of Experimental Biology·Christian De SantisMelony J Sellars

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