Expression profiling in transformed human B cells: influence of Btk mutations and comparison to B cell lymphomas using filter and oligonucleotide arrays

European Journal of Immunology
T C IslamC I Smith

Abstract

We have used both Clontech Atlas Human Hematology/Immunology cDNA microarrays, containing 588 genes, and Affymetrix oligonucleotide U95Av2 human array complementary to more than 12,500 genes to get a global view of genes expressed in Epstein-Barr virus (EBV)-transformed B cells and genes regulated by Bruton's tyrosine kinase (Btk). We compared EBV-transformed wild-type (WT) B cells from a healthy individual, WT1 and an X-linked agammaglobulinemia (XLA) patient cell line, XLA1, using the Clontech filters arrays. Eleven genes were > or =1.9-fold induced in absence of functional Btk. Furthermore, we analyzed a second patient cell line, XLA2, and compared this to two WT cell lines using oligonucleotide arrays. A total of 391 genes were found to be differentially expressed, including kinases and transcriptions factors. Furthermore, one expressed sequence tag and eight complementary DNA clones with unknown function were down-regulated in XLA2, indicating their biological role. Higher-fold inductions, Fyn (39.5), Hck (15.5) and Cyp1B1 (5.8), were observed using oligonucleotide array and were confirmed using real-time PCR for Fyn (20.8), Hck (6.7) and Cyp1B1 (10). Two genes, B cell translocation gene1 (BTG1) and B cell-specific OCT bin...Continue Reading

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Citations

Mar 3, 2004·Journal of Pharmacological and Toxicological Methods·Catherine Y S CheungThomas K H Chang
Mar 16, 2007·Assay and Drug Development Technologies·Shan JiangJ Andrew Whitney
Jul 27, 2005·Journal of Translational Medicine·Ena Wang
Oct 10, 2003·Cancer Investigation·Dezhong Joshua LiaoFazlul H Sarkar
May 2, 2006·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·T ZanderL M Nadler
Jan 23, 2003·Annals of the New York Academy of Sciences·Luc AujameMarissa Vicari
Nov 13, 2003·Current Opinion in Allergy and Clinical Immunology·Haiying QinDavid L Nelson
Jun 13, 2006·Biochemical and Biophysical Research Communications·Jessica M LindvallC I Edvard Smith

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