Expression profiling of cancerous and normal breast tissues identifies microRNAs that are differentially expressed in serum from patients with (metastatic) breast cancer and healthy volunteers.

Breast Cancer Research : BCR
Eleni van SchooneveldSteven J Van Laere

Abstract

MicroRNAs (miRNAs) are a group of small noncoding RNAs involved in the regulation of gene expression. As such, they regulate a large number of cellular pathways, and deregulation or altered expression of miRNAs is associated with tumorigenesis. In the current study, we evaluated the feasibility and clinical utility of circulating miRNAs as biomarkers for the detection and staging of breast cancer. miRNAs were extracted from a set of 84 tissue samples from patients with breast cancer and eight normal tissue samples obtained after breast-reductive surgery. After reverse transcription and preamplification, 768 miRNAs were profiled by using the TaqMan low-density arrays. After data normalization, unsupervised hierarchical cluster analysis (UHCA) was used to investigate global differences in miRNA expression between cancerous and normal samples. With fold-change analysis, the most discriminating miRNAs between both tissue types were selected, and their expression was analyzed on serum samples from 20 healthy volunteers and 75 patients with breast cancer, including 16 patients with untreated metastatic breast cancer. miRNAs were extracted from 200 μl of serum, reverse transcribed, and analyzed in duplicate by using polymerase chain r...Continue Reading

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References

Aug 30, 2000·Nature·C M PerouD Botstein
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Nov 4, 2004·Trends in Genetics : TIG·Peizhang XuBruce A Hay
Mar 16, 2005·Cell·Steven M JohnsonFrank J Slack
Jun 10, 2005·Nature·Jun LuTodd R Golub
Aug 17, 2005·Cancer Research·Marilena V IorioCarlo M Croce
Sep 6, 2005·Science·Phillip D Zamore, Benjamin Haley
Nov 29, 2005·Science·Xantha Karp, Victor Ambros
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefano VoliniaCarlo M Croce
Mar 7, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Cinzia SevignaniCarlo M Croce
Jan 4, 2007·BMC Biotechnology·Johanna RinneMaija Vihinen-Ranta
Jul 20, 2007·Molecular and Cellular Biology·Zhijun DuanMarshall S Horwitz
Sep 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Muller FabbriCarlo M Croce
Jul 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick S MitchellMuneesh Tewari
Aug 21, 2008·Clinical Genetics·A E Erson, E M Petty
Sep 6, 2008·PloS One·Shlomit GiladAyelet Chajut
Nov 13, 2008·PloS One·Melissa Piper HunterClay B Marsh
Feb 11, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Joel S ParkerPhilip S Bernard
Jun 18, 2009·Genome Biology·Pieter MestdaghJo Vandesompele
Jul 14, 2009·The Journal of Pathology·Raffaele BaffaAnne Rosenberg
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Leandro CastellanoJustin Stebbing
Feb 6, 2010·Annals of Surgery·Helen M HeneghanMichael J Kerin
Mar 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Michael L GatzaJoseph R Nevins
May 7, 2010·Journal of Cell Science·Vandhana Muralidharan-ChariCrislyn D'Souza-Schorey
May 28, 2010·Breast Cancer Research and Treatment·Hongling LiRobert Chunhua Zhao
Jul 7, 2010·Cell·Graziano MartelloStefano Piccolo

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Citations

Oct 31, 2012·Cancer Metastasis Reviews·David S GutteryJacqueline A Shaw
Oct 5, 2013·Breast Cancer Research : BCR·Heidi Schwarzenbach
Mar 9, 2013·PloS One·Rom S LeidnerCheryl L Thompson
Nov 7, 2013·PloS One·Katarina CukBarbara Burwinkel
Sep 26, 2014·BMC Veterinary Research·Magdalena HulanickaMichał Jank
Apr 11, 2015·Cancers·Ryou-U TakahashiTakahiro Ochiya
Feb 4, 2016·Indian Journal of Cancer·S Maduri
Dec 4, 2015·International Journal of Molecular Sciences·Andrea MatheKelly A Avery-Kiejda
Aug 29, 2012·International Journal of Cancer. Journal International Du Cancer·Katarina CukBarbara Burwinkel
Mar 6, 2012·Seminars in Cancer Biology·Julia KantermanMichal Baniyash
Dec 3, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Sanaa EissaHanan H Shehata
Nov 18, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhiju ZhaoShu Li
Jan 30, 2015·Biological Reviews of the Cambridge Philosophical Society·Jen N GohAlan P Kumar
Dec 3, 2014·Biochimica Et Biophysica Acta·Maša Alečković, Yibin Kang
Jan 30, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ute Warnecke-EberzElfriede Bollschweiler
Mar 4, 2015·BMC Bioinformatics·Antonino FiannacaAlfonso Urso
Oct 4, 2014·Forensic Science International. Genetics·Sarah S SilvaR Medeiros
Dec 5, 2014·Genes & Diseases·Colles Price, Jianjun Chen
Feb 28, 2015·Cancer Metastasis Reviews·Andrew McGuireMichael J Kerin
Feb 19, 2015·Biomarkers in Medicine·Samantha Khoury, Nham Tran
Sep 16, 2014·Advanced Drug Delivery Reviews·Guofeng Cheng
May 24, 2016·International Journal of Molecular Sciences·Hsiuying Wang
Jun 2, 2016·Scientific Reports·Claude Pasquier, Julien Gardès
Jun 7, 2016·Biochemical and Biophysical Research Communications·Yunda ZhangQi Luo
Sep 3, 2016·Archives of Toxicology·Vikas Ghai, Kai Wang
Sep 10, 2016·Breast Cancer Research : BCR·Bethany N HannafonW Q Ding
Jan 12, 2017·Genes·Weidan JiChangqing Su

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Methods Mentioned

BETA
PCR

Software Mentioned

RmiR
BioConductor
Affymetrix
Ingenuity Pathway Analysis ( IPA )
Ingenuity Pathway
R
limma
package
TargetScan
PicTar

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