Expression profiling of genes encoding glutamate and GABA receptor subunits in three immortalized GnRH cell lines

Brain Research
Vasilios T GaryfallouH F Urbanski

Abstract

Gonadotropin-releasing hormone (GnRH) plays a central role in regulating development and function of the reproductive axis, and its secretion is known to be influenced by glutamate and GABA. In the present study, we used gene microarrays and RT-PCR to compare the expression profiles of glutamate and GABA receptor subunits in three immortalized GnRH cell lines: GT1-1, GT1-7, and Gn10. All of these cell lines expressed the AMPA glutamate receptor subunit genes GluR2 and GluR4, but only the GT1-1 and GT1-7 cells expressed the kainate glutamate receptor subunit gene KA2. Additionally, GluRdelta2, a subunit that can form heteromeric receptors with kainate and AMPA subunits, was present in GT1-1 and Gn10 cells but not in GT1-7 cells. Genes encoding the GABA(A) receptor alpha3, beta2, beta3, epsilon, and pi subunits, as well as the GABA(B) receptor 1 subunit, were evident in all three cell lines. However, the gene encoding the expression of GABA(A) receptor gamma subunit was noticeably absent. Taken together, these data demonstrate comprehensive screening of neurotransmitter receptor genes in a controlled neuronal culture system, and reveal novel features.

References

May 1, 1994·Neuroendocrinology·G Martínez de la EscaleraR I Weiner
Sep 1, 1996·Reviews of Reproduction·H F UrbańskiV T Garyfallou
Nov 29, 2002·Molecular Endocrinology·R Anthony DeFazioSuzanne M Moenter
Feb 8, 2003·Brain Research. Molecular Brain Research·Kazuhisa KohdaMichisuke Yuzaki

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Citations

Oct 13, 2010·Molecular and Cellular Endocrinology·Virginie MansuyFrançois P Pralong
Jan 31, 2017·General and Comparative Endocrinology·Haruhiko KanasakiSatoru Kyo
Dec 17, 2009·American Journal of Physiology. Endocrinology and Metabolism·Paolo N CatalanoVictoria A Lux-Lantos
Apr 3, 2020·Cellular and Molecular Neurobiology·Marco ZattoniGiuseppe Legname

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