Abstract
Background: Diagnostic molecular marker studies are in vogue to have insight of most prevalent animal diseases including cancer. Objectives: Gene expression profiling of pro and anti-apoptotic genes was conducted in dog Lymphoma, CTVT, SCC, granuloma, perianal adenocarcinoma and mammary tumors. Materials and Methods: Cancerous tissues of 21 affected animals were obtained. Total RNA was extracted followed by cDNA synthesis. Comparative Ct method via Taqman assay (RT-qPCR) was used to quantify corresponding mRNA molecules, Tp53 and Hspb1, as normalized by GAPDH as the reference gene . Results:Hspb1 showed ectopic expression in lymphoma, CTVT and mammary tumors; its down-regulation was observed in granuloma and oral SCC with fold difference (FD) of ±35. Similarly, Tp53 as the tumor suppressor gene with pro-apoptotic properties, showed up-regulation in all tumor types, notably 80% of mammary tumors and 60% of CTVT. The FD values were 33.31 and 2.27, respectively. Conclusion: Altered transcriptomic response of Hspb1 and Tp53 was observed in all cancer types of Canis familiaris. The resulting profile depicts the involvement of the genes in cancer pathways. Thus, the data might be helpful for diagnosis, prognosis, identification and c...Continue Reading
References
Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Jan 1, 1994·Archives of Dermatological Research·W H BoehnckeW Sterry
Oct 16, 1999·Science·T R GolubE S Lander
May 16, 2000·Advances in Experimental Medicine and Biology·K V LukacsE W Alton
Dec 20, 2000·Radiation Research·K SuzukiM Watanabe
Sep 15, 2001·Veterinary Immunology and Immunopathology·R M ChuW C Lee
Oct 18, 2001·Oncology Reports·S HagaN H Sarkar
Jan 10, 2002·Microbiology·Mangala A NadkarniNeil Hunter
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 23, 2002·Progress in Molecular and Subcellular Biology·Daniel R Ciocca, Laura M Vargas-Roig
Jul 20, 2002·Veterinary Pathology·A KoenigJ F Modiano
Jul 26, 2005·Cell Stress & Chaperones·Daniel R Ciocca, Stuart K Calderwood
Nov 16, 2006·Cell Cycle·Carmen GarridoGuido Kroemer
Nov 17, 2006·Nature Reviews. Cancer·Lauren M F MerloCarlo C Maley
May 1, 2007·FEBS Letters·André-Patrick ArrigoPatrick Vicart
May 8, 2007·Trends in Cell Biology·Karim Bensaad, Karen H Vousden
May 11, 2007·BioTechniques·Amanda B HummonThomas Ried
Feb 1, 2008·The New England Journal of Medicine·Carlo M Croce
Mar 13, 2008·Cell Stress & Chaperones·Mariarita RomanucciLeonardo Della Salda
Aug 8, 2008·Placenta·P MurthiB Kalionis
Aug 18, 2009·The Veterinary Clinics of North America. Small Animal Practice·Chad Schmiedt
Aug 27, 2009·British Journal of Cancer·C S FosterJ Cuzick
Oct 17, 2009·Proteomics. Clinical Applications·Hilal ArnoukWilliam S Dynan
Oct 21, 2009·Molecular Biology Reports·Weijie LiuHuanlong Qin
Jan 13, 2011·The Laryngoscope·Julien A NortonWilliam S Dynan
Aug 13, 2011·Journal of Cancer Research and Clinical Oncology·Jörg-Christian HeinrichRudolf Fahrig
Feb 3, 2012·Molecular & Cellular Proteomics : MCP·Gu-Qing ZengZhi-Qiang Xiao
May 11, 2012·The International Journal of Biochemistry & Cell Biology·Amina Zoubeidi, Martin Gleave
Jul 19, 2012·Current Molecular Medicine·G M NagarajaA Asea
Dec 5, 2012·Vaccine·Jack CuzickAnne Szarewski
Jun 1, 2010·Surgical Pathology Clinics·Joseph A HoldenLester J Layfield