Expression profiling of MMA-1a and splice variant MMA-1b: new cancer/testis antigens identified in human melanoma.

International Journal of Cancer. Journal International Du Cancer
Nicole J W de WitGoos N P Van Muijen

Abstract

Using high-density oligonucleotide array analysis, we have recently compared the gene expression profiles of 2 human melanoma cell lines with marked difference in metastatic behavior after subcutaneous inoculation into nude mice (de Wit et al., Melanoma Res, in press). We identified an expressed sequence tag (EST), which we called malignant melanoma-associated 1 (MMA-1a), showing evident differential expression between the 2 cell lines. The MMA-1a gene is localized on chromosome 21q22.2 and its mRNA exists of 4 exons. Homology search displayed a splice variant of MMA-1a that lacks exon 3 and that was called MMA-1b. Expression profiles of MMA-1a and MMA-1b are determined by reverse transciptase polymerase chain reaction (RT-PCR) analysis. Among 30 different normal tissue samples, expression of MMA-1a and MMA-1b was exclusively found in the testis after a first PCR of 30 cycles. Even more sensitive screening achieved by performing multiple semi-nested RT-PCR showed no or very low expression in the other normal tissues tested. During melanocytic tumor progression, MMA-1a and/or MMA-1b exhibited an emergence of expression in primary melanoma (20%) and melanoma metastasis samples (30%) after only 1 round of PCR. Expression of MMA-1a...Continue Reading

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Citations

Sep 19, 2002·International Journal of Cancer. Journal International Du Cancer·Angela BarrettJoyce Taylor-Papadimitriou
Jan 13, 2006·Cancer Immunology, Immunotherapy : CII·Amanda K MilesRobert C Rees
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Jan 28, 2003·Journal of Cellular Physiology·Albert J W ZendmanGoos N P Van Muijen
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Aug 28, 2012·International Journal of Oncology·Myung-Ha SongSang-Yull Lee
Jan 7, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Takaho OkadaYutaka Kawakami

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