Expression profiling of peripheral blood miRNA using RNAseq technology in dairy cows with Escherichia coli-induced mastitis

Scientific Reports
Zhuo-Ma LuorengLin-Sen Zan

Abstract

E. coli is the main causative agent of mastitis in dairy cows, but the mechanism of molecular regulation underlying the occurrence and development of mastitis has not yet been fully elucidated. In this study, an E. coli-induced mastitis model was created and RNASeq technology was used to measure the miRNA expression profiles at different times post-infection (0, 1, 3, 5, 7 dpi), as well as to screen for differentially expressed miRNA. The results show detection of 2416 miRNAs, including 628 known miRNAs and 1788 newly discovered miRNAs. A total of 200 differentially expressed miRNAs were found at different time points. Bioinformatics analysis showed that these differentially expressed miRNAs may regulate the occurrence and development of mastitis in dairy cows through seven signal transduction pathways, namely cytokine-cytokine receptor interaction, MAPK signaling pathway, chemokine signaling pathway, leukocyte transendothelial migration, T cell receptor signaling pathway, Toll-like receptor signaling pathway, and cell adhesion molecules. In addition, bta-miR-200a, bta-miR-205, bta-miR-122, bta-miR-182 and the newly discovered conservative_15_7229 might be involved in immune process in late stage of E. coli-induced mastitis. Th...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 14, 2002·Physiological Genomics·Chiara RomualdiGian Antonio Danieli
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·John D Storey, Robert Tibshirani
Sep 24, 2004·RNA·Marc RehmsmeierRobert Giegerich
Dec 26, 2007·Nucleic Acids Research·Doron BetelChris Sander
Jul 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick S MitchellMuneesh Tewari
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Oct 12, 2010·Nature Immunology·Anna-Barbara StittrichMir-Farzin Mashreghi
Sep 14, 2011·Nucleic Acids Research·Marc R FriedländerNikolaus Rajewsky
Dec 21, 2011·Leukemia·J Contreras, D S Rao
Apr 14, 2012·The Veterinary Journal·Olga Wellnitz, Rupert M Bruckmaier
Aug 9, 2012·Theory in Biosciences = Theorie in Den Biowissenschaften·Günter P WagnerVincent J Lynch
May 25, 2013·International Forum of Allergy & Rhinology·Hille SuojalehtoHarri Alenius
Aug 8, 2013·International Journal of Molecular Sciences·Michael HeckerUwe Klaus Zettl
Jan 29, 2014·G3 : Genes - Genomes - Genetics·Nathan LawlessDavid J Lynn
Dec 17, 2014·Frontiers in Immunology·Nathan LawlessDavid J Lynn
Feb 7, 2015·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Asada LeelahavanichkulSomchai Eiam-Ong
Mar 10, 2015·International Journal of Molecular Sciences·Rui LiZhang-Ping Yang
Mar 5, 2016·BMC Genomics·Jason Ioannidis, F Xavier Donadeu
Mar 25, 2016·International Journal of Molecular Sciences·Gloria BertoliIsabella Castiglioni
Mar 31, 2016·Nature Reviews. Immunology·Ming O Li, Alexander Y Rudensky
Apr 20, 2016·International Journal of Molecular Sciences·Lijuan ZhaoLi Lin
Jun 12, 2016·Developmental and Comparative Immunology·Yanjin WangTianjun Xu
Oct 30, 2016·Molecular and Cellular Probes·Xing Ping WangShuan Liu
Feb 9, 2017·International Journal of Molecular Sciences·Martin J StoneJulie Sanchez
Jul 26, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiao-Yan WangLe-Dong Sun
Feb 28, 2018·International Journal of Biological Sciences·Zhuo-Ma LuorengLin-Sen Zan

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Citations

Mar 4, 2020·Mikrochimica Acta·Adrián Sánchez-VisedoMaría Teresa Fernández-Argüelles
Oct 3, 2020·Frontiers in Veterinary Science·Hengyu XiJingmin Gu
Aug 6, 2020·Frontiers in Genetics·Mohammad Reza BakhtiarizadehMohammad Sadegh Vafaei Sadi
Jan 5, 2021·Frontiers in Veterinary Science·Silvia MirettiMario Baratta
Nov 10, 2020·Research in Veterinary Science·Yu-Chang LaiNaoki Miura
Jun 20, 2021·Molecular Biology Reports·Zhangrui ChengUNKNOWN GplusE consortium

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Methods Mentioned

BETA
RNAseq

Software Mentioned

randfold
Bowtie
Qubit
RNAfold
RNAhybrid
miRDeep2
KOBAS
RNAseq
HemI1
miRanda

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