Expression profiling of rainbow trout testis development identifies evolutionary conserved genes involved in spermatogenesis.

BMC Genomics
Antoine D RollandF Le Gac

Abstract

Spermatogenesis is a late developmental process that involves a coordinated expression program in germ cells and a permanent communication between the testicular somatic cells and the germ-line. Current knowledge regarding molecular factors driving male germ cell proliferation and differentiation in vertebrates is still limited and mainly based on existing data from rodents and human. Fish with a marked reproductive cycle and a germ cell development in synchronous cysts have proven to be choice models to study precise stages of the spermatogenetic development and the germ cell-somatic cell communication network. In this study we used 9K cDNA microarrays to investigate the expression profiles underlying testis maturation during the male reproductive cycle of the trout, Oncorhynchus mykiss. Using total testis samples at various developmental stages and isolated spermatogonia, spermatocytes and spermatids, 3379 differentially expressed trout cDNAs were identified and their gene activation or repression patterns throughout the reproductive cycle were reported. We also performed a tissue-profiling analysis and highlighted many genes for which expression signals were restricted to the testes or gonads from both sexes. The search for ...Continue Reading

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Citations

Oct 10, 2012·Journal of Molecular Endocrinology·Elisabeth SambroniFlorence Le Gac
Jan 4, 2013·The Journal of Endocrinology·Nathalie HinfrayFrançois Brion
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Datasets Mentioned

BETA
GSE12769
E-TABM-130
GSE16029

Methods Mentioned

BETA
PCR

Software Mentioned

RepeatMasker
Excel
BZscan
Ensembl BioMart
Ensembl
AMEN
TreeView
Cluster

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