Expression profiling of single mammalian cells--small is beautiful

Yeast
G Brady

Abstract

Increasingly mRNA expression patterns established using a variety of molecular technologies such as cDNA microarrays, SAGE and cDNA display are being used to identify potential regulatory genes and as a means of providing valuable insights into the biological status of the starting sample. Until recently, the application of these techniques has been limited to mRNA isolated from millions or, at very best, several thousand cells thereby restricting the study of small samples and complex tissues. To overcome this limitation a variety of amplification approaches have been developed which are capable of broadly evaluating mRNA expression patterns in single cells. This review will describe approaches that have been employed to examine global gene expression patterns either in small numbers of cells or, wherever possible, in actual isolated single cells. The first half of the review will summarize the technical aspects of methods developed for single-cell analysis and the latter half of the review will describe the areas of biological research that have benefited from single-cell expression analysis.

References

Sep 1, 1978·Nucleic Acids Research·A E SippelG Schütz
Sep 1, 1992·Annals of Oncology : Official Journal of the European Society for Medical Oncology·L H TrümperT W Mak
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·James EberwineP Coleman
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R N Van GelderJ Eberwine
Apr 25, 1989·Nucleic Acids Research·A V BelyavskyK Rajewsky
Jan 1, 1989·Journal of Cellular Biochemistry·D A RappoleeZ Werb
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·G SauvageauR K Humphries
Oct 20, 1995·Science·V E VelculescuK W Kinzler
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·K MiyashiroJames Eberwine
Jan 1, 1993·Methods in Enzymology·G Brady, Norman N Iscove
Nov 8, 1996·Science·M R Emmert-BuckL A Liotta
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·T ChengD T Scadden
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·P B CrinoJames Eberwine
Jan 27, 1998·Genomics·J AdjayeM Monk
May 22, 1998·Journal of Cardiovascular Pharmacology·K M Schmidt-OttM Paul
Aug 6, 1998·Journal of Immunological Methods·M G KimR H Schwartz
Sep 22, 1998·Nucleic Acids Research·A K DixonT C Freeman
Jan 12, 1999·Nature Medicine·L LuoM G Erlander
Jan 23, 1999·Nature Genetics·D J DugganJeffrey M Trent
Jan 23, 1999·The American Journal of Pathology·F FendM Raffeld
Feb 19, 1999·BioTechniques·C A Suarez-QuianR F Bonner
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z BryantH Ruohola-Baker
Jun 1, 1999·Methods in Enzymology·J E KacharminaJ Eberwine
Dec 11, 1999·Brain Research. Brain Research Protocols·R Pernas-AlonsoC Perrone-Capano
Feb 26, 2000·Science·D van der Kooy, S Weiss
Feb 26, 2000·Carcinogenesis·S P BachC S Potten
Mar 18, 2000·Experimental Hematology·M V ZinovyevaA V Belyavsky
Apr 5, 2000·Nature Biotechnology·E WangF M Marincola

Citations

Oct 24, 2001·Environmental Health Perspectives·E K LobenhoferH K Hamadeh
Jan 23, 2004·Cancer Investigation·Jun LuoDavid J Duggan
Nov 17, 2007·Journal of Plant Physiology·Elke LieckfeldtJoachim Fisahn
May 1, 2007·International Journal of Medical Microbiology : IJMM·Helmy Rachman, Stefan H E Kaufmann
Aug 20, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ruben Adler
Jun 23, 2004·Annals of the New York Academy of Sciences·Ernest S Kawasaki
Aug 3, 2001·Nature Cell Biology·A Schulze, J Downward
Jun 2, 2005·Analytical and Bioanalytical Chemistry·Karen J OlsonEdgar A Arriaga
Dec 10, 2009·In Vitro Cellular & Developmental Biology. Animal·Lisa ShawSusan J Kimber
Jun 7, 2002·Endocrine Reviews·Chandra P LeoAaron J W Hsueh
May 19, 2009·Journal of Cardiovascular Medicine·Nicola MarzilianoEloisa Arbustini
Apr 23, 2008·Current Protocols in Human Genetics·Christoph A KleinNicole Wendler
Mar 27, 2003·Molecular Reproduction and Development·Anthony D MetcalfeDaniel R Brison
Feb 12, 2008·Current Protocols in Molecular Biology·Christoph A KleinNicole Wendler

Related Concepts

Poly(A) Tail
Nested Polymerase Chain Reaction
Gene Expression Regulation, Developmental
Reverse Transcriptase Polymerase Chain Reaction
MRNA Differential Display

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