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Expression profiling reveals off-target gene regulation by RNAi

Nature Biotechnology

May 20, 2003

Aimee L JacksonPeter S Linsley

Abstract

RNA interference is thought to require near-identity between the small interfering RNA (siRNA) and its cognate mRNA. Here, we used gene expression profiling to characterize the specificity of gene silencing by siRNAs in cultured human cells. Transcript profiles revealed siRNA-specific r...read more

Mentioned in this Paper

Microarray Analysis
Conserved Sequence
Transformation, Genetic
RNA, Small Interfering
Cosuppression
Molecular Profiling
Nucleotides
Gene Expression Profiling
HeLa Cells
Poly(A) Tail
1
811
1
Paper Details
References
  • References8
  • Citations1069
1
  • References8
  • Citations1069
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Expression profiling reveals off-target gene regulation by RNAi

Nature Biotechnology

May 20, 2003

Aimee L JacksonPeter S Linsley

PMID: 12754523

DOI: 10.1038/nbt831

Abstract

RNA interference is thought to require near-identity between the small interfering RNA (siRNA) and its cognate mRNA. Here, we used gene expression profiling to characterize the specificity of gene silencing by siRNAs in cultured human cells. Transcript profiles revealed siRNA-specific r...read more

Mentioned in this Paper

Microarray Analysis
Conserved Sequence
Transformation, Genetic
RNA, Small Interfering
Cosuppression
Molecular Profiling
Nucleotides
Gene Expression Profiling
HeLa Cells
Poly(A) Tail
1
811
1

Similar Papers Found In These Feeds

RNAi & Gene Silencing

RNA Interference (RNAi) pathway is involved in post-transcriptional gene silencing, transcriptional silencing and epigenetic silencing as well as its use as a tool for forward genetics and therapeutics. Discover the latest research here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America

Specificity of short interfering RNA determined through gene expression signatures

Proceedings of the National Academy of Sciences of the United States of AmericaMay 15, 2003
Dimitri SemizarovStephen W Fesik
Nature Methods

3' UTR seed matches, but not overall identity, are associated with RNAi off-targets

Nature MethodsFebruary 21, 2006
Amanda BirminghamAnastasia Khvorova
Paper Details
References
  • References8
  • Citations1069
1
  • References8
  • Citations1069
12345...
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