Expression profiling the developing mammalian enteric nervous system identifies marker and candidate Hirschsprung disease genes.

Proceedings of the National Academy of Sciences of the United States of America
Tiffany A Heanue, Vassilis Pachnis

Abstract

The enteric nervous system (ENS) is composed of neurons and glial cells, organized as interconnected ganglia within the gut wall, which controls peristalsis of the gut wall and secretions from its glands. The Ret receptor tyrosine kinase is expressed throughout enteric neurogenesis and is required for normal ENS development; humans with mutations in the RET locus have Hirschsprung disease (HSCR, an absence of ganglia in the colon), and mice lacking Ret have total intestinal aganglionosis. The Ret mutant mouse provides a tool for identifying genes implicated in development of the ENS. By using RNA from WT and Ret mutant (aganglionic) gut tissue and DNA microarrays, we have conducted a differential screen for ENS-expressed genes and have identified hundreds of candidate ENS-expressed genes. Forty-seven genes were selected for further analysis, representing diverse functional classes. We show that all of the analyzed genes are expressed in the ENS and that the screen was sensitive enough to identify genes marking only subpopulations of ENS cells. Our screen, therefore, was reliable and sensitive and has identified many previously undescribed genes for studying ENS development. Moreover, two of the genes identified in our screen Ar...Continue Reading

References

Dec 22, 1991·The Journal of Comparative Neurology·T D PhamT P Rothman
Apr 1, 1994·Cell and Tissue Research·J C LangerM G Blennerhassett
Feb 1, 1997·Current Opinion in Neurobiology·M D Gershon
Oct 10, 1997·Cell and Tissue Research·C Viollet, P Doherty
Jul 27, 1999·Developmental Biology·I T Shepherd, J A Raper
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·S BolkA Chakravarti
Jan 11, 2001·Developmental Biology·H M YoungD F Newgreen
Jun 21, 2001·Pediatric Surgery International·A YonedaP Puri
Sep 7, 2001·Current Opinion in Cell Biology·B P Liu, S M Strittmatter
Feb 22, 2002·American Journal of Medical Genetics·Melissa A ParisiKathleen A Leppig
Apr 16, 2002·Nature Genetics·Stacey B GabrielAravinda Chakravarti
May 15, 2002·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Donald Newgreen, Heather M Young
May 23, 2002·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Donald Newgreen, Heather M Young
Jun 28, 2002·Developmental Biology·Nicole Firnberg, Annette Neubüser
Nov 8, 2002·Development·Laura S Gammill, Marianne Bronner-Fraser
Dec 7, 2002·Nature Genetics·Tim O'Brien, Rick Woychik
Aug 16, 2003·Science·Toshihide IwashitaSean J Morrison
Nov 19, 2003·European Journal of Cardiovascular Nursing : Journal of the Working Group on Cardiovascular Nursing of the European Society of Cardiology·Sanne de Loor, Tiny Jaarsma
Mar 12, 2004·Cancer Letters·Masatoshi IchiharaMasahide Takahashi
Mar 23, 2004·Molecular and Cellular Neurosciences·Tam Thanh QuachPappachan E Kolattukudy
Dec 25, 2004·Clinical Genetics·A S BrooksR M W Hofstra
May 3, 2005·Cytokine & Growth Factor Reviews·Mitchell Goldfarb
May 4, 2005·Trends in Neurosciences·Vasso Episkopou
Jun 7, 2005·Nature Neuroscience·Takaharu TaketomiAkihiko Yoshimura
Aug 10, 2005·PLoS Biology·Luciano ContiAustin Smith
Aug 23, 2005·Brain Research. Brain Research Reviews·Evelyne Bloch-GallegoMatías Hidalgo-Sánchez
Sep 1, 2005·Cell and Tissue Research·R B AndersonH M Young
Oct 18, 2005·Molecular and Cellular Biology·Adrianne WongVassilis Pachnis
Dec 2, 2005·Development·Laura S GammillMarianne Bronner-Fraser

❮ Previous
Next ❯

Citations

Apr 13, 2013·Pediatric Surgery International·Weibing TangYankai Xia
Feb 9, 2012·International Journal of Colorectal Disease·Cornelia Irene HaglKarl-Herbert Schäfer
Feb 21, 2008·European Journal of Human Genetics : EJHG·Maria-Mercè Garcia-BarcelóPaul Kwong-hang Tam
Jul 23, 2004·Nature·Michael MorleyVivian G Cheung
May 22, 2007·Nature Reviews. Neuroscience·Tiffany A Heanue, Vassilis Pachnis
Feb 6, 2007·Annals of Human Genetics·M M Garcia-BarcelóP K H Tam
Apr 10, 2009·BMC Genomics·Yao Fei HuMaya Sieber-Blum
Dec 3, 2013·Orphanet Journal of Rare Diseases·Raquel M FernándezSalud Borrego
Aug 8, 2008·Development·Silvia BogniVassilis Pachnis
Feb 16, 2007·PloS One·Nikolas NikolaidisRanden L Patterson
Dec 29, 2010·PloS One·Maria M M AlvesRobert M W Hofstra
Apr 3, 2010·Human Genetics·C S TangM M Garcia-Barceló
Mar 1, 2014·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Ana TorroglosaSalud Borrego
Apr 30, 2014·Journal of Cellular Physiology·Marta RusminiIsabella Ceccherini
Sep 29, 2009·Autonomic Neuroscience : Basic & Clinical·Cátia Laranjeira, Vassilis Pachnis
Dec 24, 2015·Stem Cells International·Peter Helmut NeckelLothar Just
Jun 23, 2009·Journal of Cellular and Molecular Medicine·Marlene M Hao, Heather M Young
Apr 18, 2008·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·H M Young
Feb 14, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·G BurzynskiH Enomoto
May 8, 2013·The Journal of Comparative Neurology·Marlene M HaoHeather M Young
Mar 29, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·C HaglM Böttner
Aug 18, 2012·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·C EvangelistiE Bonora
Jun 21, 2014·Annals of Human Genetics·Hongsheng GuiMaria-Mercè Garcia-Barcelo
Jan 20, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M M HaoH M Young
Jul 26, 2012·Biochemical and Biophysical Research Communications·Tian Rui ChenFan Wang
May 7, 2013·Developmental Biology·Nadege Bondurand, Mai Har Sham
May 28, 2013·Developmental Biology·Maria M AlvesRobert M W Hofstra
Nov 6, 2012·Developmental Biology·Sonja J McKeownRichard B Anderson
Nov 23, 2007·Developmental Biology·Jean-Marie DelalandeAlan J Burns
May 28, 2016·Cell and Tissue Research·F GiancolaR Chiocchetti
Mar 24, 2015·PloS One·Caroline S HirstSonja J McKeown

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.