Expression, purification, and bioactivity of human tumstatin from Escherichia coli

Protein Expression and Purification
Quliang GuFangyu Wang

Abstract

Tumstatin is a M(r) 28,000 C-terminal NC1 fragment of type alpha3 (IV) collagen that inhibits pathological angiogenesis and suppresses proliferation of endothelial cells and growth of tumors. We report here high cytoplasmic expression of recombinant human tumstatin in Escherichia coli and its purification, in vitro refolding, and inhibitory activity analysis. Human tumstatin was expressed in the bacterial cytoplasm as an insoluble N-terminal polyhistidine tagged protein, which accounted for more than 30% of total bacterial protein in BL21 (DE3) cells. After extraction and solubilization in guanidine-HCl, recombinant protein was purified to homogeneity using a simple one-step Ni(2+)-chelate affinity chromatography and then refolded by dialysis against acidic pH buffers with gradually decreasing concentrations of denaturant. The renatured recombinant tumstatin could specifically inhibit endothelial cell proliferation in a dose-dependent manner, and suppress bFGF-induced angiogenesis in chick embryo chorioallantoic membrane and tumor growth in mouse B16 melanoma xenograft models.

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Citations

Nov 13, 2013·Protein Expression and Purification·Venugopal GundaYakkanti A Sudhakar
Mar 14, 2013·PloS One·Alen FaizJanette K Burgess
Mar 5, 2013·Biochemical and Biophysical Research Communications·Jessica ThevenardSylvie Brassart-Pasco
Aug 19, 2009·Molecular Biology Reports·Chun-xiao XuJia-xuan Xin
May 15, 2015·Technology in Cancer Research & Treatment·C WeiW S Zeng
Jul 18, 2006·Journal of Biochemistry·Jieru MengYingqi Zhang

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