Expression, purification, and crystallization of the catalytic domain of protein tyrosine phosphatase SHP-1

Journal of Structural Biology
X LiangG W Zhou

Abstract

The catalytic domain of SHP-1, a SH2-domain containing protein tyrosine phosphatase, has been crystallized by the vapor diffusion method using polyethylene glycol as the precipitant. The crystals belong to the monoclinic space group P21 with unit cell dimensions a = 42.12 A, b = 87.94 A, c = 43.22 A, alpha = 90.0 degrees, beta = 120.12 degrees, and gamma = 90.0 degrees. There is one catalytic domain of SHP-1 per asymmetric unit. X-ray was diffracted to at least 2.5 A and the crystals are appropriate for high-resolution structure determination.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Mar 11, 1994·Science·D BarfordN K Tonks
Feb 1, 1994·Trends in Genetics : TIG·G S Feng, T Pawson
Dec 1, 1993·Seminars in Cell Biology·B G Neel
Apr 1, 1996·Current Opinion in Cell Biology·M Streuli
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

Jul 12, 2002·Journal of Cellular Biochemistry·Ying QiZ Joe Zhao
Apr 6, 2011·Journal of Cellular Biochemistry·Wei WangG Wayne Zhou
Feb 15, 2005·Analytical Biochemistry·Stefan WelteNorbert Tennagels
Oct 31, 2014·Proteomics·Jianzhuo LiZhizhuang Joe Zhao
Feb 20, 1999·Journal of Cellular Biochemistry·T NiuG W Zhou
Dec 17, 2002·The Journal of Biological Chemistry·Jian YangG Wayne Zhou
Apr 28, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Suman KunduTaolin Yi
Feb 8, 2000·The Journal of Biological Chemistry·J YangG W Zhou
Oct 17, 1998·The Journal of Biological Chemistry·J YangG W Zhou
Dec 4, 2004·Journal of Cellular Biochemistry·Dandan HeG Wayne Zhou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.