PMID: 9523576Apr 2, 1998Paper

Expression, purification, and encephalitogenicity of recombinant human myelin oligodendrocyte glycoprotein

Journal of Neurochemistry
J BettadapuraC C Bernard

Abstract

Myelin oligodendrocyte glycoprotein (MOG), a putative autoantigen in multiple sclerosis (MS), is a quantitatively minor component of the CNS. In view of the difficulties associated with the purification of MOG from brain tissues, the extracellular domain of human MOG corresponding to the N-terminal 121 amino acids was expressed in Escherichia coli as a glutathione sulfotransferase fusion protein. The expressed protein was localized to inclusion bodies, and varying the growth parameters resulted in the solubilization of small amounts of GST-MOG that could be affinity purified on glutathione agarose columns. The fusion protein found in the inclusion bodies could be solubilized with urea. The solubilized fusion protein was cleaved with thrombin, and the extracellular domain was purified by CM Sephadex 50 chromatography to homogeneity. Injection of recombinant human MOG into different strains of mice resulted in the induction of an MS-like disease, characterized by severe neurological impairment and extensive CNS demyelinated lesions. Recombinant MOG produced in E. coli should prove to be useful as a highly purified biological reagent for immunological, pathological, functional, and structural studies.

Citations

Dec 24, 2013·Nature Immunology·Bryan Vander LugtHarinder Singh
Jan 14, 1999·Journal of Neurochemistry·T G Johns, C C Bernard
Jul 11, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Y OkudaT Yanagihara
Feb 17, 2001·European Journal of Immunology·M KalbusN Sommer
Feb 15, 2001·Journal of Neuroscience Research·N HellingsP Stinissen
Jan 11, 2011·Journal of Neuroimmunology·Francesca GoriFrancesco Lolli
Dec 14, 2002·Journal of the Neurological Sciences·Permphan Dharmasaroja
Feb 11, 2003·Journal of Neuroimmunology·A Kennel De MarchC C A Bernard
Jun 26, 2021·Current Protocols·Paul Smith

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