Expression, purification and preliminary crystallographic studies of human coactosin-like protein

Acta Crystallographica. Section D, Biological Crystallography
Lin LiuWeimin Gong

Abstract

Coactosin-like protein (CLP) is an actin-binding protein as well as a 5-lipoxygenase binding partner. Human coactosin-like protein has been expressed in high yield and the His-tagged protein was purified by affinity chromatography. Several different crystal forms were obtained by the hanging-drop vapour-diffusion method. X-ray diffraction data to 2.0 A resolution were collected from the best crystal. The space group was determined to be P2(1)2(1)2(1), with unit-cell parameters a = 38.4, b = 48.7, c = 72.6 A.

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Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.

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