Expression, purification and preliminary crystallographic analysis of sucrose phosphate synthase (SPS) from Halothermothrix orenii

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Frederick HuynhB K Patel

Abstract

This is the first report of the crystallization of a sucrose phosphate synthase (SPS; EC 2.4.1.14). It also constitutes the first study of a sucrose phosphate synthase from a non-photosynthetic thermohalophilic anaerobic bacterium, Halothermothrix orenii. The purified recombinant spsA protein has been crystallized in the monoclinic space group C2, with unit-cell parameters a = 154.2, b = 47.9, c = 72.3 A, beta = 103.16 degrees, using the hanging-drop vapour-diffusion method. The crystal diffracts X-rays to a resolution limit of 3.01 A. Heavy-metal and halide-soaking trials are currently in progress to solve the structure.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Apr 17, 2001·Extremophiles : Life Under Extreme Conditions·B N Mijts, B K Patel
Nov 28, 2002·Acta Crystallographica. Section D, Biological Crystallography·Nan LiKunchithapadam Swaminathan
Dec 4, 2003·Acta Crystallographica. Section D, Biological Crystallography·Tien-Chye TanKunchithapadam Swaminathan

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Citations

Jan 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Lokesh D KoriBharat K C Patel
Jan 16, 2009·PloS One·Konstantinos MavromatisNikos C Kyrpides
Sep 2, 2010·Journal of Biotechnology·Anniina T Kivistö, Matti T Karp
Feb 3, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Lokesh D KoriBharat K C Patel
Jan 13, 2015·Archives of Microbiology·Sergey Y ButYuri A Trotsenko

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