Expression, purification, crystallization and X-ray diffraction studies of a novel root-induced secreted protein from Trichoderma virens.

Acta Crystallographica. Section F, Structural Biology Communications
Ravindra BansalGagan D Gupta

Abstract

Small secreted cysteine-rich proteins (SSCPs) from fungi play an important role in fungi-host interactions. The plant-beneficial fungi Trichoderma spp. are in use worldwide as biocontrol agents and protect the host plant from soil-borne as well as foliar pathogens. Recently, a novel SSCP, Tsp1, has been identified in the secreted protein pool of T. virens and is overinduced upon its interaction with the roots of the maize plant. The protein was observed to be well conserved in the Ascomycota division of fungi, and its homologs are present in many plant-pathogenic fungi such as Fusarium oxysporum and Magnaporthe oryzae. However, none of these homologs have yet been characterized. Recombinant Tsp1 protein has been expressed and purified using an Escherichia coli expression system. The protein, with four conserved cysteines, forms a dimer in solution as observed by size-exclusion chromatography. The dimerization, however, does not involve disulfide bonds. Circular-dichroism data suggested that the protein has a β-strand-rich secondary structure that matched well with the secondary structure predicted using bioinformatics methods. The protein was crystallized using sodium malonate as a precipitant. The crystals diffracted X-rays to...Continue Reading

References

Oct 12, 2005·FEMS Microbiology Letters·Martijn Rep
Aug 15, 2006·Molecular Plant-microbe Interactions : MPMI·Slavica DjonovićCharles M Kenerley
Apr 30, 2009·Annual Review of Phytopathology·Ioannis Stergiopoulos, Pierre J G M de Wit
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
May 14, 2010·Current Biology : CB·Yariv BrotmanAda Viterbo
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Aug 7, 2013·Annual Review of Phytopathology·Prasun K MukherjeeCharles M Kenerley
Dec 3, 2013·Indian Journal of Microbiology·Mala MukherjeeSusanne Zeilinger
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
Jan 23, 2015·Frontiers in Plant Science·Ivan Baccelli
Feb 15, 2015·Molecular & Cellular Proteomics : MCP·Netta-Li LamdanBenjamin A Horwitz
May 8, 2015·Nature Protocols·Lawrence A KelleyMichael J E Sternberg
Aug 16, 2016·The New Phytologist·Pierre J G M De Wit
Apr 13, 2019·Nucleic Acids Research·Fábio MadeiraRodrigo Lopez
Jun 14, 2019·BMC Genomics·Christian P KubicekIrina S Druzhinina

❮ Previous
Next ❯

Citations

Sep 26, 2021·Proteins·Leila T AlexanderTorsten Schwede

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Virchows Archiv : an International Journal of Pathology
Jie ZhangWataru Yasui
International Journal of Cancer. Journal International Du Cancer
Salman M HyderJianbo Wu
BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology
George P Tuszynski, R F Nicosia
Experimental Biology and Medicine
I SargiannidouGeorge P Tuszynski
© 2022 Meta ULC. All rights reserved