Expression, purification, crystallization and preliminary crystallographic analysis of the PAB0955 gene product

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Stéphanie GrasDominique Housset

Abstract

PAB0955 from Pyrococcus abyssi is a prototype of a new Walker-type ATPase/GTPase conserved in archaea and eukaryota but not found in bacteria. PAB0955 has been expressed, purified and crystallized, and it has been shown that this thermostable protein is dimeric in reductive conditions. Crystals have been obtained either without nucleotide or in the presence of GDP or GTPgammaS. Preliminary X-ray crystallographic data up to 2.08 A resolution have been collected from these crystals.

Citations

Jul 20, 2011·Molecular and Cellular Biology·Clément Carré, Ramin Shiekhattar
Sep 23, 2014·FEBS Letters·Lucía E Méndez-HernándezMónica R Calera
May 1, 2007·EMBO Reports·Stéphanie GrasDominique Housset

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