Expression, purification, crystallization and preliminary crystallographic analysis of human Rad GTPase

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Arry YanuarT Hakoshima

Abstract

Human Rad is a new member of the Ras GTPase superfamily and is overexpressed in human skeletal muscle of individuals with type II diabetes. The GTPase core domain was overexpressed in Escherichia coli and purified for crystallization. Crystals were obtained at 293 K by vapour diffusion using a crystallization robot. The crystals were found to belong to space group P2(1), with unit-cell parameters a = 52.2, b = 58.6, c = 53.4 A, beta = 97.9 degrees , and contained two Rad molecules in the crystallographic asymmetric unit. A diffraction data set was collected to a resolution of 1.8 A using synchrotron radiation at SPring-8.

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Citations

Jul 27, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Arry YanuarToshio Hakoshima
Jun 23, 2006·The Journal of Biological Chemistry·Brian S FinlinDouglas A Andres

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