Expression, purification, crystallization and preliminary X-ray diffraction analysis of an essential lipoprotein implicated in cell-wall biosynthesis in Mycobacteria

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Zara MarlandJamie Rossjohn

Abstract

Mycobacterium tuberculosis is a renewed cause of devastation in the developing world. Critical to the success of this re-emerging pathogen is its unusual waxy cell wall, which is rich in rare components including lipoarabinomannan (LAM) and its precursors, the phosphatidylinositol mannosides (PIMs). Balanced synthesis of these related glycolipids is intrinsic to both cell-wall integrity and virulence in M. tuberculosis and presents a promising, albeit poorly defined, therapeutic target. Here, the expression, purification and crystallization of an essential 600-amino-acid lipoprotein, LpqW, implicated in this process are reported. Crystals of LpqW were grown using 20-24%(w/v) PEG 4000, 8-16%(v/v) 2-propanol, 100 mM sodium citrate pH 5.5 and 10 mM DTT. A complete data set was collected at 2.4 A using synchrotron radiation on a crystal belonging to space group C222, with unit-cell parameters a = 188.57, b = 312.04, c = 104.15 A. Structure determination is under way.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Jan 1, 1995·Annual Review of Biochemistry·P J Brennan, H Nikaido
Jul 18, 1997·The Journal of Biological Chemistry·G S BesraP J Brennan
Mar 27, 2003·Molecular Microbiology·Christopher M SassettiEric J Rubin
Apr 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rutilio A FrattiVojo Deretic
Nov 25, 2003·The Biochemical Journal·Yasu S MoritaMalcolm J McConville

❮ Previous
Next ❯

Citations

May 16, 2006·Journal of Molecular Biology·Zara MarlandJamie Rossjohn

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Bacterial Protein Structures (ASM)

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

© 2021 Meta ULC. All rights reserved