Expression, regulation and functional activities of aminopeptidase N (EC 3.4.11.2; APN; CD13) on murine macrophage J774 cell line

Immunobiology
Jelka GabrilovacDragomira Majhen

Abstract

Aminopeptidase N (APN; CD13) is a ubiquitous membrane-bound enzyme. Expressed on haematopoietic cells APN participates in inflammatory and immune responses by regulating local concentration of chemotactic peptides and by fine-tuning antigen presentation. The data of this study have shown for the first time that cells of murine macrophage line, J774, often used as a model cell line, express CD13 both at transcriptional level and at the level of membrane protein with aminopeptidase N (APN) activity. The level of transcriptional expression of CD13/APN on J774 cells was compared to that found on normal cells participating in immune responses. The highest CD13/APN level was found in peritoneal macrophages, followed by J774 cells and splenocytes, whereas lymph node, thymus and bone-marrow cells expressed low level of CD13/APN mRNA. Further, the CD13 (mRNA, protein and APN) on J774 cells could be up-regulated by pro-inflammatory IFN-γ which is in agreement with the known role of CD13/APN in inflammatory responses. Co-regulation of CD13 with MHC-II and CD86 is in line with the reported role of APN expressed on human cells in antigen presentation. CD13 on J774 cells co-localize with mannose receptors (MR), and co-internalize upon MR lig...Continue Reading

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Citations

Sep 26, 2013·BioMed Research International·Mónica I Villaseñor-CardosoEnrique Ortega
Feb 18, 2014·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Alexandros NikolaouJo A Van Ginderachter
Mar 4, 2017·Cell Biochemistry and Function·Emilija ZapletalJelka Gabrilovac
May 1, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mallika GhoshLinda H Shapiro
May 3, 2015·Journal of Leukocyte Biology·Ileana Licona-LimónEnrique Ortega

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