Expression status of heat shock proteins in response to cold, heat, or insecticide exposure in the Colorado potato beetle Leptinotarsa decemlineata

Cell Stress & Chaperones
Pascal DumasPier Jr Morin

Abstract

The Colorado potato beetle (Leptinotarsa decemlineata (Say)) is an agricultural pest that threatens the potato industry worldwide. This insect is widely regarded as one of the most difficult-to-control pests, as it can thrive in a wide range of temperature conditions and routinely develops resistance towards various insecticides. The molecular changes associated with response to these challenges have not been fully investigated in L. decemlineata. While differential expression and characterization of heat shock proteins (HSPs) in response to stress have been conducted in several insects, data regarding HSPs in L. decemlineata are limited. The overarching objective of this study consisted of evaluating the expression of various HSPs in L. decemlineata exposed to different temperatures or treated with the insecticides imidacloprid and chlorantraniliprole. Expression levels of HSP60, HSP70, HSP90, and HSP Beta-1 were evaluated by qRT-PCR and insect mortality was assessed using dsRNAs aimed at select HSP targets. Elevated HSP70 and HSP90 transcript levels were observed in heat-exposed L. decemlineata while downregulation of HSP70 transcript levels was measured in insects submitted to cold conditions. Chlorantraniliprole exposure wa...Continue Reading

References

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Jul 25, 2018·The Journal of Experimental Biology·Hervé ColinetKendra J Greenlee

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Citations

May 10, 2021·Ecotoxicology and Environmental Safety·Yu-Cheng WangYu-Zhou Du
May 28, 2021·Experimental and Molecular Pathology·Xia LiYi-Heng Pan

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Methods Mentioned

BETA
PCR

Software Mentioned

CFX Maestro

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