Expression studies of superoxide dismutases in nodules and leaves of transgenic alfalfa reveal abundance of iron-containing isozymes, posttranslational regulation, and compensation of isozyme activities

Molecular Plant-microbe Interactions : MPMI
Maria C RubioManuel Becana

Abstract

The composition of antioxidant enzymes, especially superoxide dismutase (SOD), was studied in one nontransgenic and three transgenic lines of nodulated alfalfa plants. Transgenic lines overproduced MnSOD in the mitochondria of nodules and leaves (line 1-10), MnSOD in the chloroplasts (line 4-6), and FeSOD in the chloroplasts (line 10-7). In nodules of line 10-7, the absence of transgene-encoded FeSOD activity was due to a lack of mRNA, whereas in nodules of line 4-6 the absence of transgene-encoded MnSOD activity was due to enzyme inactivation or degradation. Transgenic alfalfa showed a novel compensatory effect in the activities of MnSOD (mitochondrial) and FeSOD (plastidic) in the leaves, which was not caused by changes in the mRNA levels. These findings imply that SOD activity in plant tissues and organelles is regulated, at least partially, at the posttranslational level. All four lines had low CuZnSOD activities and an abundant FeSOD isozyme, especially in nodules, indicating that FeSOD performs important antioxidant functions other than the scavenging of superoxide radicals generated in photosynthesis. This was confirmed by the detection of FeSOD cDNAs and proteins in nodules of other legumes such as cowpea, pea, and soyb...Continue Reading

References

Nov 1, 1992·Free Radical Biology & Medicine·L A del RíoF J Corpas
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W Van CampD Inzé
Mar 25, 1989·Nucleic Acids Research·T C VerwoerdA Hoekema
Apr 1, 1989·European Journal of Biochemistry·G von HeijneR G Herrmann
Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Jan 1, 1984·Methods in Enzymology·H Aebi
Oct 1, 1993·The Plant Journal : for Cell and Molecular Biology·B HorvathT Bisseling
Aug 1, 1995·Computer Applications in the Biosciences : CABIOS·M G Claros
Apr 1, 1997·Plant Physiology·Y KanayamaA B Bennett
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Feb 5, 1998·Plant Physiology·D A DaltonJ M Chatfield
Mar 9, 1999·Plant Physiology·B D McKersieK S Jones
Nov 11, 1999·Plant Physiology·M A MatamorosM Becana
Jan 1, 1993·Plant Physiology·J. G. Scandalios
Aug 1, 1981·Plant Physiology·S M Bridges, M L Salin

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Citations

Dec 4, 2008·Applied Biochemistry and Biotechnology·Milen GeorgievMaria Angelova
Apr 9, 2008·Molecular Plant-microbe Interactions : MPMI·Daniel MarinoCesar Arrese-Igor
Nov 5, 2005·Applied and Environmental Microbiology·Lior WeissmanAyala Hochman
Apr 5, 2003·Physiologia Plantarum·Rapolu MadhusudhanHitoshi Shibata
Jan 8, 2015·Frontiers in Plant Science·K Judith WebbAna L Winters
Feb 26, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Mostafa M S Ismaiel, Michele D Piercey-Normore

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