Extended depth of focus in tomographic phase microscopy using a propagation algorithm

Optics Letters
Wonshik ChoiMichael S Feld

Abstract

Tomographic phase microscopy is a laser interferometry technique in which a 3D refractive index map of a biological sample is constructed from quantitative phase images collected at a set of illumination angles. Although the resulting tomographic images provide valuable information, their resolution declines at axial distances beyond about 1 microm from the focal plane. We describe an improved 3D reconstruction algorithm in which the field at the focal plane is numerically propagated to depths throughout the sample. Diffraction is thus incorporated, extending the depth of focus to more than 10 mum. Tomograms with improved focal depth are demonstrated for single HT29 cells.

Citations

Apr 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Serhan O IsikmanAydogan Ozcan
Apr 5, 2008·Blood Cells, Molecules & Diseases·Gabriel PopescuKamran Badizadegan
Feb 2, 2011·Journal of Biomedical Optics·Christopher Fang-YenMichael S Feld
May 20, 2015·Micron : the International Research and Review Journal for Microscopy·Peng XiuXu Liu
Oct 16, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Ying ZhangXin-Hua Hu
Jan 9, 2009·Optics Express·Yongjin SungMichael S Feld
Mar 26, 2014·Optics Express·Julianna KostenckaMałgorzata Kujawińska
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Oct 15, 2011·Optics Express·Zhuo WangGabriel Popescu
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Apr 5, 2012·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Colin J R SheppardChristian Depeursinge
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Apr 29, 2020·Biomedical Optics Express·Jos van Rooij, Jeroen Kalkman
Oct 17, 2008·Optics Letters·Shan Shan Kou, Colin J R Sheppard
Jul 16, 2021·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Shengli FanBahaa Saleh

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