PMID: 11331636May 2, 2001Paper

Extended embryo culture in human assisted reproduction treatments

Human Reproduction
M LangleyK M Doody

Abstract

In order to evaluate the niche of extended embryo culture in an IVF programme, retrospective analysis of non-selected IVF patients, who underwent ovarian stimulation from April 1998 to June 1999 in a single private practice assisted reproductive technology centre, was performed. Embryos were cultured for 48 h in S1/G1.2 medium followed by 48 to 72 h of culture in S2/G2.2 to day 5 or day 6. Only fertilized oocytes exhibiting two pronuclei from donor and non-donor IVF and intracytoplasmic sperm injection (ICSI) cases were examined to determine the relationship between embryo cell number on day 3 and subsequent rate of blastocyst formation. Results indicated that a proportional relationship existed between the number of blastomeres present in day 3 embryos and the rate of blastocyst formation. Fifty-four per cent of embryos that had six cells on day 3 formed blastocysts, while 76% of those embryos with eight cells formed blastocysts. Blastocyst development did not increase further when embryos had more than eight cells on day 3, indicating that embryos with greater cell numbers on day 3 are not always predictive of a greater likelihood of blastocyst formation. Fertilized oocytes exhibiting two pronuclei from donors produced signif...Continue Reading

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Citations

May 30, 2006·Cell and Tissue Banking·H W Michelmann, P Nayudu
Feb 3, 2009·Journal of Assisted Reproduction and Genetics·Seyed Noureddin Nematollahi-mahaniAmirmehdi Nematollahi-mahani
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Sep 12, 2016·Journal of Assisted Reproduction and Genetics·Daniela Paes Almeida Ferreira BragaEdson Borges

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