Extended nanofluidic immunochemical reaction with femtoliter sample volumes

Small
Kentaro ShiraiTakehiko Kitamori

Abstract

The growing need to optimize immunoassay performance driven by interest in analyzing individual cells has resulted in a decrease in the amount of sample required. Miniaturized immunoassays that use ultra-small femtoliter to attoliter sample volumes, a range known as the extended nanospace, can satisfy this analytical need; however, capturing every targeted molecule without loss in extended nanochannels for subsequent detection remains challenging. This is the first report of a successful extended nanofluidics-based quantitative immunochemical reaction capable of high capture efficiency using a femtoliter-scale sample volume. A novel patterning method using a photolithographic technique with vacuum ultraviolet light and low-temperature (100 °C) bonding enables patterning of functional groups for antibody immobilization before bonding, resulting in an immunochemical reaction space of only 86 fL. Reaction rate analyses indicate a decrease in the required sample volume to 810 fL and improvement in the limit of detection to 3 zmol, 5-6 orders of magnitude better than possible with the microfluidic immunoassay format. Highly efficient (near 100%) immunochemical reactions on a seconds time scale are possible due to the nm-scale diffus...Continue Reading

References

Jul 1, 1960·The Journal of Clinical Investigation·R S YALOW, S A BERSON
Dec 13, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·John A Howarter, Jeffrey P Youngblood
Jun 27, 2008·Analytical and Bioanalytical Chemistry·Takehiko TsukaharaTakehiko Kitamori
Feb 20, 1990·Biotechnology and Bioengineering·M T Tyn, T W Gusek
Jul 16, 2009·Analytical Chemistry·Lee A TesslerRobi D Mitra
Feb 25, 2010·Chemical Society Reviews·Takehiko TsukaharaTakehiko Kitamori
Apr 2, 2010·Lab on a Chip·M NapoliS Pennathur
Apr 28, 2010·Analytical and Bioanalytical Chemistry·Alphonsus H C NgAaron R Wheeler
Nov 9, 2010·Analytical and Bioanalytical Chemistry·Elizabeth M MillerAaron R Wheeler
Dec 3, 2011·Analytical and Bioanalytical Chemistry·Yan XuTakehiko Kitamori
Dec 14, 2011·Current Opinion in Biotechnology·Jangir SelimkhanovLev S Tsimring
Apr 17, 2012·Journal of Chromatography. a·Ryo IshibashiTakehiko Kitamori

❮ Previous
Next ❯

Citations

Oct 16, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Klaus MathwigLiza Rassaei
May 23, 2015·Biosensors & Bioelectronics·Junshan LiuLiding Wang
Jul 3, 2016·The Review of Scientific Instruments·Kavya Mohan, Partha Pratim Mondal
Mar 23, 2017·Advanced Healthcare Materials·Lei Mou, Xingyu Jiang
Mar 30, 2018·Lab on a Chip·Kazuma MawatariTakehiko Kitamori
Apr 4, 2019·Lab on a Chip·Yutaka KazoeTakehiko Kitamori
Nov 3, 2017·Advanced Materials·Yan Xu
Oct 9, 2019·The Analyst·Tatsuro NakaoTakehiko Kitamori
Jan 21, 2020·The Analyst·Stan B J WillemsVittorio Saggiomo
Feb 13, 2020·The Analyst·Tatsuro NakaoTakehiko Kitamori
Mar 28, 2019·Biomicrofluidics·Ryoichi OhtaTakehiko Kitamori
Jan 25, 2018·The Analyst·Kentaro ShiraiTakehiko Kitamori
Jan 13, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ling LinTakehiko Kitamori
Nov 7, 2014·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·J D BerryD J E Harvie
Oct 31, 2020·Analytical Chemistry·Koki YamamotoYo Tanaka
Dec 2, 2014·ACS Applied Materials & Interfaces·Chuanzhao ChenXinxin Li
Dec 15, 2016·Nano Letters·Joachim FritzscheChristoph Langhammer
Dec 29, 2020·ACS Sensors·Barbora ŠpačkováChristoph Langhammer
Jul 25, 2019·Analytical Chemistry·Yoshiyuki Tsuyama, Kazuma Mawatari
Jun 1, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hiroki SanoTakehiko Kitamori

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.