Extended Release of an Anti-Heparan Sulfate Peptide From a Contact Lens Suppresses Corneal Herpes Simplex Virus-1 Infection

Investigative Ophthalmology & Visual Science
Dinesh JaishankarDeepak Shukla

Abstract

To prolong the release of a heparan sulfate binding peptide, G2-C, using a commercially available contact lens as a delivery vehicle and to demonstrate the ability of the released peptide to block herpes simplex virus-1 (HSV-1) infection using in vitro, ex vivo, and in vivo models of corneal HSV-1 infection. Commercially available contact lenses were immersed in peptide solution for 5 days prior to determining the release of the peptide at various time points. Cytotoxicity of the released samples was determined by MTT and cell cycle analysis, and the functional activity of the released samples were assessed by viral entry, and viral spread assay using human corneal epithelial cells (HCE). The ability to suppress infection in human and pig cornea ex vivo and mouse in vivo models were also assessed. Peptide G2-C was released through the contact lens. Following release for 3 days, the peptide showed significant activity by inhibiting HSV-1 viral entry and spread in HCE cells. Significant suppression of infection was also observed in the ex vivo and in vivo experiments involving corneas. Extended release of an anti-HS peptide through a commercially available contact lens can generate significant anti-HSV-1 activity and provides a n...Continue Reading

Citations

Apr 27, 2017·Current Eye Research·Ştefan ŢăluStefano Giovanzana
Feb 16, 2018·Science Translational Medicine·Dinesh JaishankarDeepak Shukla
Dec 18, 2018·Frontiers in Pharmacology·Dominik D KaltenbachVaibhav Tiwari
Sep 25, 2017·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Alex Hui
Oct 18, 2018·Cellular and Molecular Life Sciences : CMLS·Lulia KoujahDeepak Shukla
Aug 28, 2019·Science Advances·Tejabhiram YadavalliDeepak Shukla
Jun 21, 2020·Journal of Clinical Medicine·David WuDeepak Shukla
Jan 1, 2021·Pharmaceutics·N ToffolettoA P Serro
Oct 30, 2019·Microorganisms·Raghuram KogantiDeepak Shukla
Nov 12, 2020·The Ocular Surface·Onkar B SawantShahzad I Mian
Feb 9, 2021·Experimental Eye Research·Raghuram KogantiAfsar R Naqvi
Feb 5, 2021·Frontiers in Cellular and Infection Microbiology·Krishnaraju MadavarajuDeepak Shukla
Feb 12, 2017·Biomaterials·Guoting QinChengzhi Cai
Aug 28, 2020·Acta Biomaterialia·Xin FanAllan E David
Mar 30, 2021·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Lyndon JonesMark Willcox
Jul 3, 2021·Pathogens·Rahul K SuryawanshiDeepak Shukla
Jun 17, 2021·Bioscience Reports·Safa KinanehShadi Hamoud

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Methods Mentioned

BETA
FACS
fluorescence-activated cell sorting
Infection

Software Mentioned

GraphPad Prism
ImageQuant
MetaMorph
Axiovision
GraphPad

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