Sep 18, 2012

Extending the viability of emu spermatozoa during in vitro storage by manipulation of temperature and diluent potassium concentration

British Poultry Science
S SoodG B Martin

Abstract

1. Survival of emu spermatozoa during in vitro storage is not affected by increasing the extracellular [K(+)] to the point where it does not adversely affect spermatozoa function. 2. In three experiments, the effects were studied of [K(+)] in a diluent in the range 12·5-80 mM/l on emu spermatozoa survival for up to 48 h at 5, 10 or 20°C. 3. At the end of the storage period, spermatozoa viability, motility, fertilising ability and morphology were measured. 4. In Experiment 1, spermatozoa viability and morphology were adversely affected after storage (P < 0·001) only in the diluent containing 80 mM/l [K(+)] whereas spermatozoa motility decreased as [K(+)] increased from 12·5 to 80 mM/l. 5. In Experiment 2, during storage at 5°C, the spermatozoa viability was comparable among any of the diluents (standard or modified) but morphology was better (P < 0·001) in all of the modified diluents than in the standard E3 diluent. 6. In Experiment 3, after 48 h of storage in a diluent containing 40 mM/l of [K(+)], the spermatozoa functions were better preserved at 10°C than at 5 or 20°C. 7. It is concluded that a higher than physiological level of potassium can be used in a diluent without detrimental effect on emu spermatozoa survival during...Continue Reading

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Citations

Mentioned in this Paper

Ovum
Extracellular
Cell Motility
Science of Morphology
Potassium
Sperm Cell
Spermatozoa Cell Count
Motility
Biologic Preservation
Specimen Collection

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