Extensive amyloid formation in transplanted microencapsulated mouse and human islets

Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis
Sara Bohman, G T Westermark

Abstract

Deposition of cell toxic islet amyloid is a frequent finding in type 2 diabetes and also in transplanted human islets, where it is a possible explanation for their long-term failure. One suggested reason for amyloid in transplanted islets is that their low vascular density results in a disturbed local clearance of islet amyloid polypeptide (IAPP). To test this hypothesis we analysed accumulation of amyloid in microencapsulated islets, which exemplify a non-vascularised islet graft. Isolated islets from human or transgenic mice expressing human IAPP were microencapsulated in alginate and cultured in vitro or transplanted under the kidney capsule of normoglycemic nude mice. The degree of amyloid was determined after Congo red staining and subcellular alterations were analysed with electron microscopy. Insulin and IAPP secretion from transgenic mouse islets were markedly increased during stimulation with glucose after one week of culture, but encapsulated islets in general released less insulin. Amyloid was detected after both one and three weeks of culture in the transgenic mouse islets and the encapsulated islets were most affected. After transplantation, electron microscopy displayed both intra- and extracellular amyloid in mic...Continue Reading

References

Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P WestermarkC Betsholtz
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P WestermarkK H Johnson
Nov 14, 1986·Biochemical and Biophysical Research Communications·P WestermarkK Sletten
Jan 1, 1972·Upsala Journal of Medical Sciences·P Westermark
Nov 1, 1965·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H Puchtler, F Sweat
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·E J de KoningJ S Verbeek
Jan 26, 1996·The Journal of Biological Chemistry·T A MirzabekovB L Kagan
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·C B VerchereS E Kahn
Jan 1, 1996·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A ClarkE J de Koning
Jun 1, 1997·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·C B VerchereS E Kahn
Feb 26, 1999·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·E J de KoningA Clark
Apr 30, 2002·Diabetes·Göran MattssonPer-Ola Carlsson
Oct 23, 1964·Science·T M CHANG
Jan 16, 2007·Biochemical and Biophysical Research Communications·Sakeneh ZraikaSteven E Kahn
Aug 30, 2008·The New England Journal of Medicine·Gunilla T WestermarkUNKNOWN Nordic Network for Clinical Islet Transplantation
Oct 28, 2008·Biochemical and Biophysical Research Communications·Sara Bohman, Aileen J F King
Nov 12, 2010·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Marie DavalRalf Langen
Apr 14, 2011·Upsala Journal of Medical Sciences·Per Westermark
Jun 10, 2011·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Anat Frydman-MaromEhud Gazit
Jul 12, 2011·Physiological Reviews·Per WestermarkGunilla T Westermark
Dec 24, 2011·Transplantation·Gunilla T WestermarkPer Westermark

❮ Previous
Next ❯

Citations

Dec 31, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Per-Ola CarlssonOlle Korsgren
Aug 16, 2017·Journal of Molecular Endocrinology·Daniel RaleighAnne Clark
Jan 31, 2018·Journal of Molecular Endocrinology·Heather C Denroche, C Bruce Verchere
May 11, 2017·European Journal of Endocrinology·Daniel EspesPer-Ola Carlsson
Nov 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Alexander Ulrich ErnstMinglin Ma
Nov 19, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Freya Van HulleDaniel Pipeleers
Jun 12, 2021·Frontiers in Endocrinology·Kristel ParvGustaf Christoffersson

❮ Previous
Next ❯