PMID: 9530994Apr 8, 1998Paper

Extensive binding of the bioflavonoid quercetin to human plasma proteins

The Journal of Pharmacy and Pharmacology
D W BoultonThomas Walle

Abstract

Although the bioflavonoids, a large group of polyphenolic natural products, exert chemopreventive effects in cardiovascular disease and cancer, there is little information about the disposition of these dietary components in man. The objective of this study was to investigate the plasma-protein binding of the most abundant bioflavonoid, quercetin, using 14C-labelled quercetin. An ultracentrifugation assay (170,000 g for 16 h at 20 degrees C) was shown to sediment plasma proteins. Binding of quercetin to normal plasma was extensive (99.1+/-0.5%, mean +/- s.d., n = 5). The unbound fraction varied as much as 6-fold, 0.3-1.8%, between subjects. This high binding was independent of quercetin concentration over the range 1.5-15 microM (0.5-5 microg mL(-1)). Human serum albumin was the primary protein responsible for the binding of quercetin in plasma (99.4+/-0.1%). Binding by alpha1-acid glycoprotein (39.2+/-0.5%) and very-low-density lipoproteins (<0.5% of total quercetin) did not make substantial contributions to overall plasma binding. The equilibrium association constant for the binding of quercetin to serum albumin was 267+/-33 x 10(3) M(-1) (n = 15). Thermodynamic data for the binding of quercetin to serum albumin indicated spo...Continue Reading

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Citations

Mar 21, 2007·Pharmaceutical Research·Trudy Rodgers, Malcolm Rowland
Mar 17, 2004·Free Radical Biology & Medicine·Thomas Walle
May 17, 2000·European Journal of Pharmacology·Y MitsunagaY Sawada
Jun 1, 2000·Free Radical Biology & Medicine·P AderS Wolffram
Nov 9, 2000·The Journal of Steroid Biochemistry and Molecular Biology·Y OtakeT Walle
Apr 16, 2013·Journal of Agricultural and Food Chemistry·Christopher KropatDoris Marko
Oct 25, 2008·Critical Reviews in Food Science and Nutrition·Gregory Tucker, Kevin Robards
Jun 26, 2002·Biological & Pharmaceutical Bulletin·Ulrich Kragh-HansenMasaki Otagiri
Oct 27, 2004·Bioscience, Biotechnology, and Biochemistry·Trevor M Kitson
Oct 25, 2001·Analytical Biochemistry·B MayerA Hermetter
May 16, 2014·Nutrition Reviews·Torsten Bohn
Jan 1, 2014·High Altitude Medicine & Biology·Jyotsna PrasadShashi Bala Singh
Mar 22, 2001·British Journal of Clinical Pharmacology·T WalleP V Halushka
Jul 19, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Magalí Sáez-AyalaJosé Neptuno Rodríguez-López
Jun 2, 2007·Expert Opinion on Drug Metabolism & Toxicology·Thomas WalleU Kristina Walle
Mar 8, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Kathrin ManderyHartmut Glaeser
Mar 23, 2011·The Journal of Nutritional Biochemistry·Eileen LayMirna Z Ilic
May 20, 2008·Journal of Ethnopharmacology·Mei YaoPatricia D Brown-Woodman
Sep 20, 2015·Neurochemistry International·Seyed Fazel NabaviSeyed Mohammad Nabavi
Jan 25, 2006·British Journal of Pharmacology·Lee JiaJoseph E Tomaszewski
Mar 22, 2016·Nutrients·Yao LiYulong Yin
Mar 7, 2006·Chemico-biological Interactions·Hester van der WoudeIvonne M C M Rietjens
Nov 26, 2002·Biochemical and Biophysical Research Communications·Bidisa Sengupta, Pradeep K Sengupta
Jun 6, 2006·Biochimica Et Biophysica Acta·Meng-Xia XieYing-Dian Wang
Jun 1, 2005·Biochimica Et Biophysica Acta·Meng-Xia XieYing-Dian Wang

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