Extensive mis-splicing of a bi-partite plant mitochondrial group II intron.

Nucleic Acids Research
Helen Elina, Gregory G Brown

Abstract

Expression of the seed plant mitochondrial nad5 gene involves two trans-splicing events that remove fragmented group II introns and join the small, central exon c to exons b and d. We show that in both monocot and eudicot plants, extensive mis-splicing of the bi-partite intron 2 takes place, resulting in the formation of aberrantly spliced products in which exon c is joined to various sites within exon b. These mis-spliced products accumulate to levels comparable to or greater than that of the correctly spliced mRNA. We suggest that mis-splicing may result from folding constraints imposed on intron 2 by base-pairing between exon a and a portion of the bi-partite intron 3 downstream of exon c. Consistent with this hypothesis, we find that mis-splicing does not occur in Oenothera mitochondria, where intron 3 is further fragmented such that the predicted base-pairing region is not covalently linked to exon c. Our findings suggest that intron fragmentation may lead to mis-splicing, which may be corrected by further intron fragmentation.

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Citations

Mar 2, 2011·Nucleic Acids Research·Kerstin PetersenRalph Bock
Jan 4, 2013·PloS One·Jean-Claude FarréBenoît Castandet
Mar 21, 2012·The Plant Journal : for Cell and Molecular Biology·Ido KerenOren Ostersetzer-Biran
Jun 1, 2011·The Plant Journal : for Cell and Molecular Biology·Kristina KühnJames Whelan
May 13, 2017·The International Journal of Biochemistry & Cell Biology·Matthew NguLinda Bonen

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

mfold

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