Extensive translatome remodeling during ER stress response in mammalian cells.

PloS One
Iván VentosoJavier Santoyo

Abstract

In this work we have described the translatome of two mammalian cell lines, NIH3T3 and Jurkat, by scoring the relative polysome association of ∼10,000 mRNA under normal and ER stress conditions. We have found that translation efficiencies of mRNA correlated poorly with transcript abundance, although a general tendency was observed so that the highest translation efficiencies were found in abundant mRNA. Despite the differences found between mouse (NIH3T3) and human (Jurkat) cells, both cell types share a common translatome composed by ∼800-900 mRNA that encode proteins involved in basic cellular functions. Upon stress, an extensive remodeling in translatomes was observed so that translation of ∼50% of mRNA was inhibited in both cell types, this effect being more dramatic for those mRNA that accounted for most of the cell translation. Interestingly, we found two subsets comprising 1000-1500 mRNA whose translation resisted or was induced by stress. Translation arrest resistant class includes many mRNA encoding aminoacyl tRNA synthetases, ATPases and enzymes involved in DNA replication and stress response such as BiP. This class of mRNA is characterized by high translation rates in both control and stress conditions. Translation i...Continue Reading

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Citations

Feb 9, 2016·Cell Reports·J J David HoStephen Lee
Apr 21, 2016·ASN Neuro·Nicolò MusnerLawrence Wrabetz
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Mar 12, 2021·Wiley Interdisciplinary Reviews. RNA·J J David HoPhilip A Marsden

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Datasets Mentioned

BETA
GSE36206
GSE36207

Methods Mentioned

BETA
environmental stress
RNAseq
chips
protein folding
environmental stresses
electrophoresis
PCR

Software Mentioned

Ensembl
Agilent
FatiGO
Excel
JMP8
FatiScan
Babelomics

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