Extent and Mode of Action of Cationic Legume Proteins against Listeria monocytogenes and Salmonella Enteritidis

Probiotics and Antimicrobial Proteins
Mahmoud Z SitohyAly Al-Gaby

Abstract

The methylated soybean protein and methylated chickpea protein (MSP and MCP) with isoelectric points around pI 8 were prepared by esterifying 83 % of their free carboxyl groups and tested for their interactions with Listeria monocytogenes and Salmonella Enteritidis. The two substances exhibited a concentration-dependent inhibitory action against the two studied bacteria with a minimum inhibitory concentration of about 100 μg/mL. The IC50 % of the two proteins against L. monocytogenes (17 μg/mL) was comparable to penicillin but comparatively much lower (15 μg/mL) than that of penicillin (85 μg/mL) against S. Enteritidis. The two proteins could inhibit the growth of L. monocytogenes and S. Enteritidis by about 97 and 91 %, respectively, after 6-12 h of incubation at 37 °C. The constituting subunits of MSP (methylated 11S and methylated 7S) were both responsible for its antimicrobial action. Transmission electron microscopy of the protein-treated bacteria showed various signs of cellular deformation. The cationic proteins can electrostatically and hydrophobically interact with cell wall and cell membrane, producing large pores, pore channels and cell wall and cell membrane disintegration, engendering higher cell permeability leadi...Continue Reading

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Citations

Feb 8, 2020·Journal of the Science of Food and Agriculture·Entsar AbbasMahmoud Sitohy
Jul 8, 2020·Journal of Food Science·Hitomi SasakiMakoto Kanauchi
Mar 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seham Abdel-ShafiMahmoud Sitohy
Nov 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seham Abdel-ShafiAli Osman
Feb 26, 2020·Animals : an Open Access Journal From MDPI·Ruixue WangLi Yi
Dec 17, 2020·Antibiotics·Abdul-Raouf Al-MohammadiMohamed A Taha
Jan 23, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ali OsmanMahmoud Sitohy
Aug 7, 2021·Antibiotics·Ali OsmanNashwa El-Gazzar

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