PMID: 2498487Apr 1, 1989Paper

Extent of formation of a dimeric adenine photoproduct in polynucleotides and DNA

Journal of Photochemistry and Photobiology. B, Biology
N D Sharma, R J Davies

Abstract

Quantum yields are reported for the formation of a dimeric adenine photoproduct, A = A, in adenine homopolymers and DNA irradiated at 254 nm. The A = A content of irradiated samples was assayed by using reversed-phase HPLC to isolate the 4,6-diamino-5-guanidinopyrimidine (DGPY) which is produced from A = A on acid hydrolysis. Acid hydrolysates derived from DNA radiolabelled with [14C] 2'-deoxyadenosine were spiked with unlabelled DGPY before fractionation on HPLC and the recovered material was further purified by chromatography on Sephadex G-10 followed by co-crystallization with DGPY sulphate. Although A = A is formed with a relatively high quantum yield of 1.6 X 10(-3) mol einstein-1 in single-stranded poly(dA) the photoaddition reaction is strongly quenched in base-paired poly(dA).poly(dT) and undetectable in poly(rA).poly(dT). Respective quantum yields of 6 X 10(-5) and 9 X 10(-6) were estimated for the formation of A = A in single- and double-stranded E. coli DNA implying that the photoproduct has very limited biological significance. From studies with d(ApG), d(GpA), ApG, GpA, d(A)20 and d(A4G)4 it is concluded that adjacent guanine and adenine bases do not form a photoadduct analogous to A = A and also that guanine resid...Continue Reading

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Citations

Oct 31, 2001·Journal of Photochemistry and Photobiology. B, Biology·J L RavanatJ Cadet
Feb 1, 1994·Photochemistry and Photobiology·V HejmadiR J Davies
Jul 12, 2012·Photochemistry and Photobiology·Jean CadetThierry Douki
Dec 21, 2013·Photochemistry and Photobiology·Andrea Christa KneuttingerThomas Carell
May 11, 2016·The Journal of Physical Chemistry Letters·Akos BanyaszRoberto Improta
Apr 11, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Thierry Douki
Jan 7, 2011·Journal of Nucleic Acids·Rajesh P RastogiRajeshwar P Sinha
Feb 28, 2013·Photochemistry and Photobiology·Sindhu G Nair, Glen R Loppnow
Feb 1, 1995·Redox Report : Communications in Free Radical Research·N KrishnamacharyN J Duker
Nov 1, 1994·Journal of Photochemistry and Photobiology. B, Biology·H Görner

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