PMID: 7536132Jun 1, 1994Paper

Extent of RNA editing of glutamate receptor subunit GluR5 in different brain regions of the rat

Cellular and Molecular Neurobiology
W Paschen, B Djuricic

Abstract

1. The structure and function of glutamate receptor subunits GluR2, GluR5, and GluR6 are changed by RNA editing. This reaction produces a base transition in the second transmembrane spanning region. The triplet CAG (coding for glutamine) is changed to CGG (coding for arginine). This transition has a pronounced effect on calcium fluxes through the respective ion channels, because calcium currents decrease with the rate of editing. 2. In the present study the extent of RNA editing of the glutamate receptor subunit GluR5 was studied in different brain regions of control rats using a newly developed analysis system. This system is based on restriction analysis of the polymerase chain reaction (PCR) product, derived from reverse-transcribed mRNA as template, with the enzyme Bbv1. Bbv1 recognizes the sequence of the nonedited receptor subunit around the edited base (sequence GCAGC) but not that of the edited subunit (sequence GCGGC; A edited to G). 3. Total RNA was isolated from the cerebral cortex, striatum, hippocampus, thalamus, hypothalamus, cerebellum, pons/medulla oblongata, and white matter and reverse transcribed into cDNA. The region across the edited sequence was amplified by PCR using GluR5-specific primers and the cDNA as...Continue Reading

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Citations

Dec 22, 1999·Analytical Biochemistry·H H Schiffer, S F Heinemann
Mar 1, 1997·Somatic Cell and Molecular Genetics·L VillardK Gardiner
Dec 30, 2004·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Shin Kwak, Yukio Kawahara
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Feb 20, 1997·Brain Research. Developmental Brain Research·W PaschenE Dux
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Jul 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·W PaschenA Uto
Dec 11, 2002·Proceedings of the National Academy of Sciences of the United States of America·Samuel KarlinMichael Cleary
May 26, 2004·Neuroscience Letters·Núria de VeraEmili Martínez
Apr 8, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Wulf PaschenThorsten Mengesdorf
Jan 24, 2007·Journal of Neurochemistry·Douglas P OlsenMichele H Jacob
Dec 11, 1997·The Journal of Experimental Zoology·E K Hoffmann

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