PMID: 11897844Mar 19, 2002Paper

External anions and cations distinguish between AMPA and kainate receptor gating mechanisms

The Journal of Physiology
Derek Bowie

Abstract

Experiments were designed to examine if ion-flow through alpha-amino-3-hydroxy-5-methyl-isoxazolepropionic acid (AMPA) or kainate receptors interferes with protein structures associated with the gating machinery. Gating was studied using ultra-fast drug perfusion of outside-out patches containing rat GluR-A or GluR6 subunits excised from transfected human embryonic kidney cells. Deactivation rates of GluR6 kainate receptors observed following brief L-glutamate (10 mM Glu, 1 ms) applications differed by two to threefold in high (405 mM symmetrical Na(+), tau(decay) = 2.7 ms at -100 mV) and low ionic strength (55 mM, tau(decay) = 1.1 ms) solutions. In comparison, GluR-A AMPA receptors were much less sensitive. Ion effects on GluR6 receptors did not reflect surface potential screening or ion-agonist competition at the agonist-binding site since deactivation rates were slower in high ionic strength solutions. Moreover, the apparent agonist affinity did not decrease with increasing ionic strength (e.g. 55 mM, EC(50) = 110 microM vs. 405 mM, EC(50) = 61 microM). GluR6 responses were strongly dependent on ions present on the external, but not the internal, side of the plasma membrane. Decay kinetics was regulated by the type of ion pr...Continue Reading

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Citations

Jul 19, 2006·Cell and Tissue Research·Paulo Pinheiro, Christophe Mulle
Aug 21, 2013·Nature Structural & Molecular Biology·G Brent DaweDerek Bowie
Nov 23, 2006·Nature Structural & Molecular Biology·Matthew C WestonMark L Mayer
Jul 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Charu ChaudhryMark L Mayer
Jan 12, 2012·Cold Spring Harbor Perspectives in Biology·Trevor G Smart, Pierre Paoletti
Jun 30, 2009·Molecular Pharmacology·Naushaba NayeemTim Green
Aug 19, 2010·Pharmacological Reviews·Stephen F TraynelisRay Dingledine
Jan 30, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kasper B HansenStephen F Traynelis
Jan 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Max H NanaoSenyon Choe
Oct 14, 2009·The Journal of Physiology·Derek Bowie
Feb 4, 2003·The Journal of Physiology·Derek BowieG David Lange
Jan 28, 2006·The Journal of Physiology·Anne-Marie L Fay, Derek Bowie
May 15, 2012·Public Health·G McCartneyG D Batty
Apr 10, 2007·Current Opinion in Neurobiology·Kasper B HansenStephen F Traynelis
Jul 1, 2011·The European Journal of Neuroscience·Pamela Lachance-TouchettePatrick Cossette
Aug 19, 2015·The Journal of Physiology·Theanne N Griffith, Geoffrey T Swanson
Oct 22, 2013·Neuron·Juan Lerma, Joana M Marques
Mar 16, 2007·Neuron·Andrew J R Plested, Mark L Mayer
Sep 17, 2008·Neuropharmacology·David E JaneGraham L Collingridge
Aug 9, 2008·Neuropharmacology·Andrew J R Plested, Mark L Mayer
Jun 14, 2008·Neuron·Andrew J R PlestedMark L Mayer
Mar 4, 2009·Biophysical Journal·Ranjit VijayanPhilip C Biggin
Jul 16, 2015·PloS One·Jerónimo A AuzmendiLuciano Moffatt
Aug 3, 2016·Journal of Chemical Information and Modeling·Maria Musgaard, Philip C Biggin
Dec 10, 2017·The Journal of General Physiology·Patricia M G E BrownDerek Bowie
Jan 8, 2010·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Sang-Bok LeeKyung-Nyun Kim
Jun 5, 2003·Nature Reviews. Neuroscience·Juan Lerma
Oct 14, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Anabel Pérez-GómezAntonello Novelli
Sep 14, 2018·F1000Research·Marco Arieli Herrera-Valdez
Jan 6, 2015·The Journal of Physiology·G Brent DaweDerek Bowie
Feb 9, 2021·Experimental Biology and Medicine·Sheila Ms Sears, Sandra J Hewett
Apr 3, 2021·Neuropharmacology·Mark L Mayer
Aug 27, 2021·Membranes·Cuauhtemoc U GonzalezVasanthi Jayaraman

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