External K+ modulates the activity of the Arabidopsis potassium channel SKOR via an unusual mechanism.

The Plant Journal : for Cell and Molecular Biology
Ingela JohanssonIngo Dreyer

Abstract

Plant outward-rectifying K+ channels mediate K+ efflux from guard cells during stomatal closure and from root cells into the xylem for root-shoot allocation of potassium (K). Intriguingly, the gating of these channels depends on the extracellular K+ concentration, although the ions carrying the current are derived from inside the cell. This K+ dependence confers a sensitivity to the extracellular K+ concentration ([K+]) that ensures that the channels mediate K+ efflux only, regardless of the [K+] prevailing outside. We investigated the mechanism of K+-dependent gating of the K+ channel SKOR of Arabidopsis by site-directed mutagenesis. Mutations affecting the intrinsic K+ dependence of gating were found to cluster in the pore and within the sixth transmembrane helix (S6), identifying an 'S6 gating domain' deep within the membrane. Mapping the SKOR sequence to the crystal structure of the voltage-dependent K+ channel KvAP from Aeropyrum pernix suggested interaction between the S6 gating domain and the base of the pore helix, a prediction supported by mutations at this site. These results offer a unique insight into the molecular basis for a physiologically important K+-sensory process in plants.

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Citations

Jun 11, 2009·The Journal of Biological Chemistry·Dietmar GeigerRainer Hedrich
Jul 26, 2008·Journal of Experimental Botany·Mark W SzczerbaHerbert J Kronzucker
Oct 10, 2008·Annual Review of Physiology·John M WardJulian I Schroeder
Dec 3, 2013·Journal of Experimental Botany·Isabelle ChérelHervé Sentenac
Oct 24, 2013·Journal of Experimental Botany·Frans J M Maathuis
Feb 18, 2016·Trends in Plant Science·Rainer HedrichIngo Dreyer
Apr 10, 2007·FEBS Letters·Anne LebaudyHervé Sentenac
Feb 13, 2014·The Plant Journal : for Cell and Molecular Biology·Cornelia EisenachMichael R Blatt
Jan 23, 2016·Plant Science : an International Journal of Experimental Plant Biology·Devrim CoskunHerbert J Kronzucker
Jun 23, 2009·Trends in Plant Science·Ingo Dreyer, Michael R Blatt
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Oct 24, 2008·Current Opinion in Plant Biology·Christopher Grefen, Michael R Blatt
Dec 17, 2008·The New Phytologist·Anna Amtmann, Michael R Blatt
Apr 21, 2006·The Plant Journal : for Cell and Molecular Biology·Kun LiuSheng Luan
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Oct 1, 2011·The FEBS Journal·Ingo Dreyer, Nobuyuki Uozumi
Jun 3, 2015·The Plant Journal : for Cell and Molecular Biology·Guangzhe YangYanhua Su
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Apr 2, 2014·Journal of Plant Physiology·Vadim Demidchik
May 9, 2014·Journal of Plant Physiology·Begoña BenitoIngo Dreyer
Feb 1, 2017·The Plant Journal : for Cell and Molecular Biology·Taiyu ChenYongjun Lin
Mar 17, 2017·Scientific Reports·Kamil SklodowskiIngo Dreyer
Jun 24, 2017·The New Phytologist·Ingo DreyerJanin Riedelsberger
Jan 24, 2019·The Plant Journal : for Cell and Molecular Biology·Limin WangHervé Sentenac
Oct 18, 2012·Physiological Reviews·Rainer Hedrich
Jul 22, 2019·Journal of Experimental Botany·Jérémy VilletteIsabelle Gaillard
Jan 21, 2020·The Plant Journal : for Cell and Molecular Biology·Alice DrainHervé Sentenac
Mar 11, 2020·Annual Review of Plant Biology·Tracy Lawson, Jack Matthews
Dec 3, 2016·The Plant Genome·Cheng ZhangChen Wang

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