PMID: 3760814Sep 1, 1986Paper

External Na-independent Ca extrusion in cultured ventricular cells. Magnitude and functional significance

The Journal of General Physiology
W H BarryJ H Bridge

Abstract

The relative magnitudes and functional significance of Ca extrusion by Na-Ca exchange and by an Nao-independent mechanism were investigated in monolayer cultures of chick embryo ventricular cells. Abrupt exposure of cells in 0-Nao, nominally 0-Cao solution to 20 mM caffeine produced a large contracture (3.94 +/- 0.90 micron of cell shortening) that relaxed with a t1/2 of 8.60 +/- 1.22 s. An abrupt exposure to caffeine plus 140 mM Na resulted in a contracture that was smaller in amplitude (1.53 +/- 0.50 micron) and relaxed much more rapidly (t1/2 = 0.77 +/- 0.09 s). An abrupt exposure to caffeine in 0-Nao solutions produced an increase in 45Ca efflux that persisted for 20 s, and a net loss of Ca content, determined by atomic absorption spectroscopy (AAS), of approximately 4 nmol/mg protein, within 35 s. A comparable net loss of Ca was demonstrated in the presence of 100 microM [Ca]o. The abrupt exposure of cultured cells to 0 Nao in 1.8 mM Ca produced a Ca uptake, estimated with 45Ca, of 3.2 nmol/mg protein X 15 s, but produced no increase in cell Ca content (AAS). In cells in which a 30% increase in Nai was produced by 5 min exposure to 10(-6) M ouabain, the abrupt exposure to 0 Nao produced a Ca uptake of 6 nmol/mg protein X 1...Continue Reading

Citations

Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·R Volkmann
May 1, 1991·Trends in Cardiovascular Medicine·W H Barry
Jan 1, 1991·Annals of the New York Academy of Sciences·S C O'NeillD A Eisner
Dec 22, 1999·The Japanese Journal of Physiology·H IshidaH Nakazawa
Feb 1, 1994·Cell Calcium·W Vierling, A Stampfl
Mar 29, 2016·Geriatrics & Gerontology International·Yoshitaka Kondo, Akihito Ishigami
Sep 1, 1987·Journal of Cardiac Surgery·W H Barry
Dec 10, 1999·Biophysical Journal·E ChudinB Kogan
Sep 29, 2000·Circulation Research·W H Barry
Nov 1, 1987·Circulation Research·W H BarryM J Cunningham
Feb 1, 1991·Circulation·R A QuaifeW H Barry
Feb 26, 2000·In Vitro Cellular & Developmental Biology. Animal·K M CrossC W Bowers
Aug 16, 1996·The Journal of Biological Chemistry·G F WangF E Stockdale
Aug 13, 1999·The American Journal of Physiology·J P GaughanS R Houser
Mar 29, 2005·Molecular and Cellular Biochemistry·Amritlal MandalSajal Chakraborti
Feb 1, 1989·The American Journal of Physiology·G A PeetersW H Barry
Oct 1, 1998·Biochemical and Biophysical Research Communications·T FujitaN Maruyama

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