External quality assessment for the detection of blood-borne viruses in plasma by nucleic acid amplification technology: the first human immunodeficiency virus and hepatitis B virus studies (HIV EQA/1 and HBV EQA/1) and the fifth hepatitis C virus study (HCV EQA/5)

Vox Sanguinis
G PisaniEQA Participants

Abstract

This External Quality Assessment (EQA) study was aimed at assessing the proficiency of blood centres and blood product manufacturers in detecting, by nucleic acid amplification technology (NAT), the possible contamination of plasma with hepatitis C virus (HCV), human immunodeficiency virus (HIV) and hepatitis B virus (HBV). Three independent panels, one for each virus, were prepared at the Istituto Superiore di Sanità (ISS) by diluting the respective reference preparations. NAT methods used by the EQA participants included polymerase chain reaction (PCR) assays by Roche, transcription-mediated amplification (TMA) assays by Chiron and in-house PCR assays. Forty-three of the 45 participants (95.6%) in the HCV EQA/5 who used a validated method were consistently able to detect a nominal concentration of 100 IU/ml for all six major genotypes. In the case of the HIV EQA/1, all 35 participants detected the samples containing 1000 IU/ml HIV, while five (14.3%) did not identify the samples containing 100 IU/ml HIV. With respect to the HBV EQA/1, all 16 participants correctly identified the positive samples containing either 1000 IU/ml or 100 IU/ml HBV. No false-positive results were observed with any of the three panels. The HCV EQA/5 s...Continue Reading

Citations

Oct 22, 2008·Transfusion Medicine·G Rodríguez LombardiM S Vitali
Aug 12, 2015·Blood·Flore Sicre de FontbruneUNKNOWN French Severe Chronic Neutropenia Registry
Aug 7, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Lunan WangJinming Li
Jan 31, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J K PreiksaitisUNKNOWN American Society of Transplantation Infectious Diseases Community of Practice
Oct 27, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Masataka TsugeKatsutoshi Yoshizato
Mar 17, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paula C SotoAjit Varki

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