External Quality Assessment Scheme for HIV-1 Drug-Resistance Genotyping in Thailand

AIDS Research and Human Retroviruses
Siriphan Saeng-AroonKazushi Motomura

Abstract

The efficacy of antiretroviral (ARV) therapy can be compromised by the emergence and transmission of HIV-1 drug-resistant strains. HIV-1 drug-resistance (DR) genotypic testing thus plays an important role in the selection of optimal treatment regimens for HIV-infected individuals. Given the complexities of the testing procedures and the variety of approaches used, there is considerable potential for results to vary between laboratories. In Thailand, the national External Quality Assessment (EQA) scheme assesses the DR genotype testing performance of laboratories. Here, we evaluated the performance of laboratories in nucleotide sequencing and compared drug-resistance-associated mutations (DRMs) in the HIV-1 protease (PR) and reverse transcriptase (RT) genes during 2010-2015. The EQA samples in the 12 panels showed predominance for the CRF01_AE (85%) and subtype B (15%). Fourteen laboratory datasets were generated: eight using TruGene (TG), two using ViroSeq (VS), and four using in-house (IH) assays. All IH and VS laboratories had penalty scores <7, whereas five of the eight TG laboratories had fluctuating penalty scores. Moreover, seven and six TG laboratories could not amplify the two identical samples, 10B and 10E samples, or ...Continue Reading

References

Feb 28, 2002·AIDS Research and Human Retroviruses·Rob SchuurmanUNKNOWN ENVA Working Group
Dec 24, 2005·Journal of Medical Virology·Diane DescampsDominique Costagliola
Nov 4, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·A PanditR Schuurman
May 11, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Neil ParkinSilvia Bertagnolio
Apr 25, 2015·AIDS Research and Therapy·Weerawat ManosuthiUNKNOWN Adults and Adolescents Committee of the Thai National HIV Guidelines Working Group
Jun 24, 2017·Annals of Laboratory Medicine·Frank SchneiderRichard C Friedberg

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Methods Mentioned

BETA
genotyping
Assay
PCR

Software Mentioned

IH
VQA
TG
Excel

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