PMID: 2110507May 4, 1990Paper

Extinction of an immunoglobulin kappa promoter in cell hybrids is mediated by the octamer motif and correlates with suppression of Oct-2 expression

Cell
S JunkerP Matthias

Abstract

When immunoglobulin-expressing B cells are fused with fibroblasts, immunoglobulin expression is rapidly and selectively suppressed. here we demonstrate that the conserved octamer motif of a kappa light chain gene promoter plays a crucial role in mediating this "extinction" phenomenon. Replacement of this octamer site by an Sp1 or NF1 binding site is sufficient to bypass extinction. Furthermore, in early cell hybrids, immunoglobulin suppression is correlated with absence of the cell-specific transcription factor Oct-2 and its transcripts. Such hybrids cannot support transcription of a transiently introduced reporter plasmid, driven by an octamer-containing promoter, unless an expression vector encoding Oct-2 is cotransfected. Transfection of the same Oct-2 expression vector into hybrid cells is also sufficient to "reactivate" an integrated kappa promoter construct. Thus, our data further establish the role of Oct-2 for immunoglobulin transcription and show that in B cell x fibroblast hybrids, the lack of a necessary cell-specific transcription factor is involved in the extinction of immunoglobulin expression.

References

Jan 1, 1989·Experimental Cell Research·S JunkerD Picard
Aug 11, 1989·Nucleic Acids Research·P MatthiasW Schaffner
Jan 1, 1988·CRC Critical Reviews in Biochemistry·B Wasylyk
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J Mizushima-Sugano, R G Roeder
Oct 28, 1988·Science·P A Baeuerle, D Baltimore
Dec 1, 1988·Genes & Development·R G ClercP A Sharp
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C PetitM C Weiss
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M C Weiss, M Chaplain
May 1, 1982·Experimental Cell Research·S Junker
Mar 11, 1983·Nucleic Acids Research·H Luthman, G Magnusson
Jun 1, 1984·Molecular and Cellular Biology·C Queen, J Stafford
Nov 1, 1983·Developmental Biology·D V DeLeonR C Angerer
Oct 15, 1981·Biochemical and Biophysical Research Communications·S Junker, S Pedersen
Dec 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·E H SZYBALSKA, W SZYBALSKI

❮ Previous
Next ❯

Citations

Jan 1, 1996·Somatic Cell and Molecular Genetics·T P Powers, R L Davidson
May 20, 1998·Biotherapy·M C Magli
Jan 1, 1993·Immunologic Research·J G Johnson, M K Jenkins
Feb 8, 2000·The International Journal of Biochemistry & Cell Biology·S MassaP Matthias
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·E AltiokI Ernberg
Dec 1, 1993·Current Opinion in Cell Biology·M H Baron
Aug 31, 2010·Genomics·Gary A BullaSharon Hickman
Jun 9, 2005·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Benjamin Bonavida, Mario I Vega
Sep 15, 2005·Bioscience Reports·Gary A Bulla, David M Kraus
Jun 28, 1996·The Journal of Biological Chemistry·H ChenD G Tenen
Oct 1, 1991·Molecular and Cellular Biology·C L MillerH Singh
Jul 1, 1992·Molecular and Cellular Biology·A AnnweilerT Wirth
Jun 1, 1993·The Journal of Investigative Dermatology·L A CorneliusS W Caughman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.