Extra- and intracellular distribution of cytokinins in the leaves of monocots and dicots

New Biotechnology
Eva JiskrováIvo Frébort

Abstract

The plant hormones cytokinins are a convenient target of genetic manipulations that bring benefits in biotechnological applications. The present work demonstrates the importance of the subcellular compartmentalization of cytokinins on the model dicot plant Arabidopsis thaliana and monocot crop Hordeum vulgare. The method of protoplast and vacuole isolation combined with precise cytokinin analysis and recovery assay of a vacuolar marker protein were used to quantify the contents of individual cytokinin forms in the leaf extracellular space, cell interior and vacuole. The data obtained for wild type plants and in each case a specific mutant line allow comparing the effect of genetic manipulations on the hormone distribution and homeostatic balance of cytokinins in the modified plants.

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Citations

Apr 1, 2016·Plant Physiology and Biochemistry : PPB·David ZalabákPetr Galuszka
Sep 8, 2016·Frontiers in Plant Science·Mária ŠmehilováPetr Galuszka
Feb 23, 2017·Annual Review of Plant Biology·Ondřej NovákKarin Ljung
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Jun 26, 2020·Frontiers in Plant Science·Klára Hoyerová, Petr Hošek
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Apr 29, 2018·Plant Physiology·Jan ŠimuraOndřej Novák
Aug 28, 2021·International Journal of Molecular Sciences·Martin RasporTatjana Ćosić

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