Extra-column dispersion of macromolecular solutes in aqueous-phase size-exclusion chromatography

Journal of Chromatography. a
G GrznárováT Görner

Abstract

A set of dextran standards was used to study the extra-column dispersion in conventional chromatographic equipment at a broad range of molecular weights, different mobile phase flow rates and connecting tube lengths and diameters. All known correlations for the tube dispersion at laminar flow, including those for short tubes, overestimated the values of the variance of the outlet concentration signal. The difference increased with the solute molecular weight and the flow rate. It was assumed that the discrepancy was due to the effect of natural convection invoked by the density differences of the injected dextran solutions and water. A suitable approximation of the relative band spreading was suggested in a form of a power function of the Reynolds and Schmidt numbers. A significant decrease of the dispersion was observed when the chromatography tubing was coiled into a circle. This decrease was successfully predicted combining the existing correlations for long coiled tubes and short straight tubes.

References

Oct 30, 2001·Journal of Chromatography. a·J P BusnelT Chang
Jan 1, 1981·Talanta·J T VandersliceD J Higgs

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Citations

Jan 5, 2005·Journal of Chromatography. a·Simona-Tereza PopoviciPeter J Schoenmakers
Dec 20, 2016·Journal of Pharmaceutical and Biomedical Analysis·Alexandre GoyonSzabolcs Fekete
Mar 15, 2018·Scientific Reports·George Cătălin MarinescuAnca Dinischiotu
Feb 5, 2013·Journal of Liquid Chromatography & Related Technologies·Paula HongEdouard S P Bouvier
Nov 4, 2017·Journal of Pharmaceutical and Biomedical Analysis·Szabolcs Fekete, Davy Guillarme
Jun 25, 2018·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Alexandre GoyonDavy Guillarme
Feb 27, 2010·Analytical Chemistry·Véronique WernertRenaud Denoyel

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