Extra-facile chiral separation of amino acid enantiomers by LC-TOFMS analysis

Journal of Bioscience and Bioengineering
Yutaka KonyaEiichiro Fukusaki

Abstract

An extra-facile chiral liquid chromatography-time of flight mass spectrometry (LC-TOFMS) analytical method of amino acid enantiomers has been developed without a derivatization process. The enantioseparation of eighteen proteinogenic amino acids (except for proline) was simultaneously performed using a combination of a chiral column (CROWNPAK CR-I(+)) and a TOFMS within 15 min. An isocratic condition of a simple mobile phase comprising acetonitrile/water/trifluoroacetic acid (96/4/0.5) gave baseline separation of all underivatized amino acid enantiomers on the chiral column.

References

Apr 11, 1969·Science·J J Corrigan
May 11, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yoshitaka GogamiTadao Oikawa
Feb 22, 2012·Journal of Pharmaceutical and Biomedical Analysis·István IliszAntal Péter
Mar 6, 2012·Journal of Pharmaceutical and Biomedical Analysis·Yurika MiyoshiKenji Hamase

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Citations

Aug 21, 2016·Journal of Bioscience and Bioengineering·Yosuke NakanoEiichiro Fukusaki
Aug 21, 2018·Frontiers in Microbiology·Hironaga AkitaToshihisa Ohshima
Jul 17, 2020·Amino Acids·Giacomo CarenziLoredano Pollegioni
Jan 23, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Atsushi OhnishiKanji Nagai
Apr 17, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Izuru KawamuraHisako Sato
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Róbert BerkeczIstván Ilisz

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