Abstract
T cell development depends on continuous recruitment of progenitors from bone marrow (BM) to the thymus via peripheral blood. However, both phenotype and functional characteristics of physiological T cell precursors remain ill-defined. Here, we characterized a putative CD135(+)CD27(+) T cell progenitor population, which lacked expression of CD127, CD90, and high levels of CD117 and was therefore termed triple negative precursor (TNP). TNPs were present in both BM and blood and displayed robust T lineage potential, but virtually no myeloid or B lineage potential, in vitro. However, TNPs did not efficiently generate T lineage progeny after intravenous or intrathymic transfer, suggesting that a physiological thymic microenvironment does not optimally support T cell differentiation from TNPs. Thus, we propose that physiological T cell precursors are confined to populations expressing either CD127, CD90, or high levels of CD117 in addition to CD135 and CD27 and that TNPs may have other physiological functions.
References
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·S Dejbakhsh-Jones, S Strober
Mar 27, 2001·Biochemical and Biophysical Research Communications·W DengD J Prockop
Aug 28, 2002·Immunity·Hideya IgarashiPaul W Kincade
Dec 14, 2002·Immunity·Thomas M Schmitt, Juan Carlos Zúñiga-Pflücker
Apr 9, 2004·Blood·S Scott PerryGerald J Spangrude
Jun 11, 2004·Immunity·Helen E PorrittHoward T Petrie
Jul 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susan E Prockop, Howard T Petrie
Aug 10, 2004·Nature Immunology·Benjamin A Schwarz, Avinash Bhandoola
Apr 27, 2005·Cell·Jörgen AdolfssonSten Eirik W Jacobsen
May 10, 2005·Nature Immunology·Fabio M V RossiHermann J Ziltener
Dec 13, 2005·Nature Immunology·Florence LambolezSophie Ezine
Dec 31, 2005·Blood·Floor WeerkampFrank J T Staal
Apr 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·M Lucila ScimoneUlrich H von Andrian
Jul 19, 2006·The Journal of Experimental Medicine·Andreas KruegerHarald von Boehmer
Aug 29, 2006·Nature Medicine·Johannes L ZakrzewskiMarcel R M van den Brink
Jan 16, 2007·Immunity·Andreas Krueger, Harald von Boehmer
Mar 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Oliver UmlandHoward T Petrie
Jun 22, 2007·Immunity·Avinash BhandoolaJuan Carlos Zúñiga-Pflücker
Aug 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laetitia GautreauSophie Ezine
Apr 1, 2008·Nature Biotechnology·Johannes L ZakrzewskiMarcel R M van den Brink
Apr 11, 2008·Nature·J Jeremiah Bell, Avinash Bhandoola
Apr 11, 2008·Nature·Haruka WadaHiroshi Kawamoto
Oct 18, 2008·Blood·Thomas SerwoldIrving L Weissman
Dec 8, 2009·Blood·Daniel A ZlotoffAvinash Bhandoola
Dec 17, 2009·Blood·Namita SaranAndreas Krueger
Dec 31, 2009·Blood·Andreas KruegerReinhold Förster
Mar 23, 2010·Immunity·Susan M SchlennerHans-Reimer Rodewald
Jul 3, 2010·Science·Tomokatsu IkawaHiroshi Kawamoto
Dec 18, 2010·Blood·Lauren I Richie EhrlichIrving L Weissman
Jul 28, 2011·Blood·Anthony W S ChiAvinash Bhandoola
Jul 28, 2011·European Journal of Immunology·Andreas Krueger