Extracellular alpha-galactosidase from Debaryomyces hansenii UFV-1 and its use in the hydrolysis of raffinose oligosaccharides

Journal of Agricultural and Food Chemistry
Pollyanna A VianaValéria Monteze Guimarães

Abstract

Raffinose oligosaccharides (RO) are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. ROs are hydrolyzed by alpha-galactosidases that cleave alpha-1,6-linkages of alpha-galactoside residues. The objectives of this study were the purification and characterization of extracellular alpha-galactosidase from Debaryomyces hansenii UFV-1. The enzyme purified by gel filtration and anion exchange chromatographies presented an Mr value of 60 kDa and the N-terminal amino acid sequence YENGLNLVPQMGWN. The Km values for hydrolysis of pNP alphaGal, melibiose, stachyose, and raffinose were 0.30, 2.01, 9.66, and 16 mM, respectively. The alpha-galactosidase presented absolute specificity for galactose in the alpha-position, hydrolyzing pNPGal, stachyose, raffinose, melibiose, and polymers. The enzyme was noncompetitively inhibited by galactose (Ki = 2.7 mM) and melibiose (Ki = 1.2 mM). Enzyme treatments of soy milk for 4 h at 60 degrees C reduced the amounts of stachyose and raffinose by 100%.

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Citations

Jun 11, 2008·Applied Biochemistry and Biotechnology·S K ShankarV H Mulimani
Feb 19, 2011·Applied Biochemistry and Biotechnology·Joana Gasperazzo FerreiraSebastião Tavares de Rezende
Oct 22, 2008·Applied Biochemistry and Biotechnology·Daniel Luciano FalkoskiSebastião Tavares de Rezende
Dec 17, 2015·International Journal of Molecular Sciences·Weiwei ZhangTzi Bun Ng
Oct 7, 2008·Phytochemistry·Lílian da Silva FialhoSebastião Tavares de Rezende
Aug 14, 2013·Critical Reviews in Biotechnology·Priti KatroliaZhengqiang Jiang
May 28, 2011·Biotechnology Progress·José Luis CorcheroAntonio Villaverde
Jul 21, 2020·Applied Microbiology and Biotechnology·Miriam AnguloCarlos Angulo
Feb 20, 2009·Journal of Agricultural and Food Chemistry·Pollyanna A VianaValéria M Guimarães
Jan 20, 2010·International Journal of Biological Macromolecules·Pollyanna A VianaValéria M Guimarães
Dec 21, 2006·Journal of Agricultural and Food Chemistry·Daniel L FalkoskiSebastião T de Rezende

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