Extracellular amastigote-like forms of Leishmania panamensis and L. braziliensis. II. Stage- and species-specific monoclonal antibodies.

The Journal of protozoology
S Eperon, D McMahon-Pratt

Abstract

Immunochemical evidence, employing monoclonal antibodies, shows that the forms of L. braziliensis complex axenically grown at elevated temperature are amastigote-like. The monoclonal antibodies were raised against membrane proteins of amastigote-like forms, strains of both L. panamensis (WR442) and L. braziliensis (M5052), which were grown axenically. The specificities of these antibodies were examined by indirect radioimmune binding assay, indirect immunofluorescent assay and Western blot analyses. Two distinct groups of monoclonal antibodies were obtained and their specificities were consistent with the 3 methods used. Four antibodies are specific for the species L. panamensis and react with both developmental stages. Six antibodies specifically recognize amastigote-like forms grown at elevated temperature and intracellular amastigotes of both L. panamensis (WR442) and L. braziliensis (M5052). These monoclonal antibodies do not bind to promastigotes of these species, nor to promastigotes of any other species of Leishmania. Therefore these antibodies are specific for amastigotes of L. panamensis (WR442) and L. braziliensis (M5052), and suggest that immunochemically both amastigote forms (culture and macrophage) are development...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1988·The American Journal of Tropical Medicine and Hygiene·S MartinezJ J Marr
Apr 1, 1988·The Journal of Cell Biology·M C BonaldoS Goldenberg
Aug 1, 1988·Experimental Parasitology·E RondinelliF T de Castro
Feb 1, 1987·Experimental Cell Research·J F CarvalhoF T de Castro
May 1, 1987·The Journal of protozoology·J J Blum
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F Lawrence, M Robert-Gero
Jan 1, 1985·Molecular and Biochemical Parasitology·V T ContrerasS Goldenberg
Jun 21, 1985·Science·L H Van der PloegC R Cantor
Jul 1, 1984·The Journal of Cell Biology·G T Williams
Dec 14, 1984·Biochemical and Biophysical Research Communications·K W HunterE G Hayunga
Nov 1, 1981·Molecular and Biochemical Parasitology·D T HartG H Coombs
Jan 1, 1964·Advances in Parasitology·S ADLER

❮ Previous
Next ❯

Citations

Mar 1, 1991·Molecular and Biochemical Parasitology·D ZilbersteinC L Jaffe
Nov 1, 1991·Molecular and Biochemical Parasitology·P M RaineyA A Pan
Oct 1, 1993·Molecular and Biochemical Parasitology·D Zilberstein, A Gepstein
Apr 1, 1994·Molecular and Biochemical Parasitology·K R Dell, J N Engel
Apr 5, 2001·Trends in Parasitology·N GuptaA K Rastogi
Aug 1, 1996·Tropical Medicine & International Health : TM & IH·N GuptaA K Rastogi
Oct 1, 1991·Experimental Parasitology·P S DoyleD M Dwyer
Jan 26, 1995·Molecular and Cellular Biochemistry·J J CastillaA Osuna
Nov 25, 2017·Cell Stress & Chaperones·Francehuli DaggerCarlos Ayesta
Mar 1, 1993·The Journal of Eukaryotic Microbiology·A A PanD McMahon-Pratt
May 1, 1994·The Journal of Eukaryotic Microbiology·J J Blum, F R Opperdoes
Sep 10, 2010·European Journal of Immunology·Tiago M CastilhoDiane McMahon-Pratt
Apr 1, 1993·Parasitology Today·P A Bates

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.