Abstract
Immunochemical evidence, employing monoclonal antibodies, shows that the forms of L. braziliensis complex axenically grown at elevated temperature are amastigote-like. The monoclonal antibodies were raised against membrane proteins of amastigote-like forms, strains of both L. panamensis (WR442) and L. braziliensis (M5052), which were grown axenically. The specificities of these antibodies were examined by indirect radioimmune binding assay, indirect immunofluorescent assay and Western blot analyses. Two distinct groups of monoclonal antibodies were obtained and their specificities were consistent with the 3 methods used. Four antibodies are specific for the species L. panamensis and react with both developmental stages. Six antibodies specifically recognize amastigote-like forms grown at elevated temperature and intracellular amastigotes of both L. panamensis (WR442) and L. braziliensis (M5052). These monoclonal antibodies do not bind to promastigotes of these species, nor to promastigotes of any other species of Leishmania. Therefore these antibodies are specific for amastigotes of L. panamensis (WR442) and L. braziliensis (M5052), and suggest that immunochemically both amastigote forms (culture and macrophage) are development...Continue Reading
References
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 1, 1978·European Journal of Biochemistry·M Dejter-JuszynskiN Sharon
Nov 1, 1986·The Journal of protozoology·K M WilliamsR L Anthony
Sep 1, 1989·The Journal of protozoology·S Eperon, D McMahon-Pratt
Mar 1, 1988·The American Journal of Tropical Medicine and Hygiene·S MartinezJ J Marr
May 1, 1987·The Journal of protozoology·F P KeeganJ J Blum
Aug 1, 1988·Experimental Parasitology·D R Taylor, G T Williams
Apr 1, 1988·The Journal of Cell Biology·M C BonaldoS Goldenberg
Aug 1, 1988·Experimental Parasitology·E RondinelliF T de Castro
Feb 1, 1987·Experimental Cell Research·J F CarvalhoF T de Castro
May 1, 1987·The Journal of protozoology·J J Blum
Jun 1, 1987·Molecular and Biochemical Parasitology·D LegrandA Capron
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F Lawrence, M Robert-Gero
Jan 1, 1985·Molecular and Biochemical Parasitology·V T ContrerasS Goldenberg
Jun 21, 1985·Science·L H Van der PloegC R Cantor
Aug 15, 1970·Nature·U K Laemmli
Jul 1, 1984·The Journal of Cell Biology·G T Williams
Dec 14, 1984·Biochemical and Biophysical Research Communications·K W HunterE G Hayunga
Aug 1, 1984·Experimental Parasitology·B D HansenM G Pappas
Nov 1, 1981·Molecular and Biochemical Parasitology·D T HartG H Coombs
Sep 12, 1980·Science·K P Chang
Jan 1, 1964·Advances in Parasitology·S ADLER
Citations
Mar 1, 1991·Molecular and Biochemical Parasitology·D ZilbersteinC L Jaffe
Nov 1, 1991·Molecular and Biochemical Parasitology·P M RaineyA A Pan
Oct 1, 1993·Molecular and Biochemical Parasitology·D Zilberstein, A Gepstein
Apr 1, 1994·Molecular and Biochemical Parasitology·K R Dell, J N Engel
Jan 1, 1993·Biology of the Cell·S EperonB Swiderski
May 1, 1997·Molecular and Biochemical Parasitology·M A SantosR L Tarleton
Apr 5, 2001·Trends in Parasitology·N GuptaA K Rastogi
Oct 1, 1992·Parasitology·P A BatesG H Coombs
Nov 1, 1994·Parasitology·S EreshD C Barker
Aug 1, 1996·Tropical Medicine & International Health : TM & IH·N GuptaA K Rastogi
Oct 13, 2001·Infection and Immunity·M ColmenaresD McMahon-Pratt
Dec 1, 1991·Acta Tropica·D R Taylor, G T Williams
Oct 1, 1991·Experimental Parasitology·P S DoyleD M Dwyer
Jan 26, 1995·Molecular and Cellular Biochemistry·J J CastillaA Osuna
Nov 13, 1995·FEBS Letters·D Sarkar, A Bhaduri
Nov 25, 2017·Cell Stress & Chaperones·Francehuli DaggerCarlos Ayesta
Mar 1, 1993·The Journal of Eukaryotic Microbiology·A A PanD McMahon-Pratt
May 1, 1994·The Journal of Eukaryotic Microbiology·J J Blum, F R Opperdoes
Sep 1, 1989·The Journal of protozoology·S Eperon, D McMahon-Pratt
Dec 5, 2003·Parasitology·T G V SilveiraA H Straus
Sep 10, 2010·European Journal of Immunology·Tiago M CastilhoDiane McMahon-Pratt
May 1, 1993·Infection and Immunity·C L BarbiériE N Figueiredo
Apr 1, 1993·Parasitology Today·P A Bates