PMID: 7544427Jan 1, 1995Paper

Extracellular antigens of Actinobacillus actinomycetemcomitans Y4 in severe alveolar bone loss patients studied by two-dimensional electrophoresis and western blots

Microbiology and Immunology
Y TaniI Kato

Abstract

The human immune response to extracellular substances (ES) from Actinobacillus actinomycetemcomitans Y4 were analyzed. Twenty-nine periodontal patients with generalized severe alveolar bone loss and 13 healthy volunteers were examined for serum IgG antibody titers against ES. Among the patients, 17 had higher antibody titers than healthy individuals (high-titer patients) but the remainder (low-titer patients) did not. Two-dimensional electrophoresis (2DE) and Western blots demonstrated that two proteins (40 and 37 kDa) and three smeared substances reacted with IgGs from high-titer patients, but not with IgGs from healthy volunteers. The low-molecular-mass smear at the acid side reacted with over 90% of all patients. This smear reacted with anti-A. actinomycetemcomitans lipopolysaccharide (LPS) monoclonal antibody which recognized LPS from each A. actinomycetemcomitans serotype. The high molecular mass smear at the acid side might be serotype-specific O-antigens of LPS. Another high molecular mass smear which located from the alkaline to the neutral side reacted with anti-serotype-b-specific capsular polysaccharide monoclonal antibody. Moreover, the 40- and 37-kDa proteins reacted with this anti-capsular polysaccharide antibody....Continue Reading

References

Mar 1, 1977·Journal of Periodontal Research·M G Newman, S S Socransky
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 1, 1992·Scientific American·E T Rietschel, H Brade
Aug 1, 1991·Infection and Immunity·D H MeyerP M Fives-Taylor
Jan 1, 1990·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J L Ebersole
Jul 1, 1982·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I M VarndellJ M Polak
Jul 1, 1982·Infection and Immunity·A NowotnyF Sanavi

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Citations

Aug 11, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M A CurtisM Rangarajan
Jul 15, 2017·Journal of Proteome Research·Arancha Llama-PalaciosMariano Sanz

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