Extracellular ATP activates the PLC/PKC/ERK signaling pathway through the P2Y2 purinergic receptor leading to the induction of early growth response 1 expression and the inhibition of viability in human endometrial stromal cells

Cellular Signalling
Shu-Ju ChangChen-Jei Tai

Abstract

ATP is an extracellular signaling molecule that activates specific G protein-coupled P2Y receptors in most cell types to mediate diverse biological effects. ATP has been shown to activate the phospholipase C (PLC)/diacylglycerol/protein kinase C (PKC) pathway in various systems. However, little is known about the signaling events in human endometrial stromal cells (hESCs). The objective of this study was to examine the presence of the P2Y2 receptor and the effects of exogenous ATP on the intracellular mitogen-activated protein kinases (MAPKs) signaling pathway, immediate early genes expression, and cell viability in hESCs. Western blot analysis, gene array analysis, and MTT assay for cell viability were performed. The current study demonstrated the existence of the P2Y2 purinergic receptor in hESCs. UTP and ATP activated MAPK in a dose- and time-dependent manner. Suramin (a P2-purinoceptor antagonist), neomycin (a PLC inhibitor), staurosporin (a PKC inhibitor), and PD98059 (a MEK inhibitor) significantly attenuated the ATP-induced activation of MAPK. ATP activated ERK1/2 and induced translocation of activated ERK1/2 to the nucleus. The gene array for 23 genes associated with members of the mitogenic pathway cascade and immediat...Continue Reading

Citations

Dec 12, 2012·Purinergic Signalling·Elisabet AliagasMireia Martín-Satué
Jun 8, 2013·Nucleosides, Nucleotides & Nucleic Acids·Ortal DaninoBilha Fischer
Nov 26, 2013·Purinergic Signalling·Geoffrey Burnstock
Jun 22, 2010·Biochimica Et Biophysica Acta·Natalia BuzziAna Russo de Boland
Dec 27, 2015·Seminars in Cell & Developmental Biology·Joell L Solan, Paul D Lampe
Jan 10, 2012·British Journal of Pharmacology·Jing-Bo ChenGui-Rong Li
Apr 20, 2011·Periodontology 2000·Prasit Pavasant, Tussanee Yongchaitrakul
Apr 27, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yanghui XingJun You
May 20, 2015·Autonomic Neuroscience : Basic & Clinical·George I Gorodeski
Dec 1, 2012·Biochemical and Biophysical Research Communications·Youn Ho ShinJunyang Jung
Apr 19, 2013·American Journal of Physiology. Cell Physiology·Ruhul AminHatim A Hassan
Sep 22, 2017·Pharmacological Reviews·Luc LeybaertRainer Schulz
Dec 11, 2019·Cell Proliferation·Xiao-Wei GuZeng-Ming Yang
Mar 10, 2020·Journal of Cellular and Molecular Medicine·Svetlana SemenovaYuri Negulyaev
Dec 16, 2016·Journal of Gastroenterology and Hepatology·Liping WuHiroto Miwa
Sep 5, 2019·Proceedings of the National Academy of Sciences of the United States of America·Saisai ChenMaciej T Nogalski
Apr 9, 2017·Archives of Biochemistry and Biophysics·Felipe R TeixeiraMarcelo D Gomes

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