Extracellular ATP modulates [Ca2+]i in retinoic acid-treated embryonic chondrocytes

The American Journal of Physiology
C T HungI M Shapiro

Abstract

When treated with low doses of retinoic acid (RA), cephalic chondrocytes of the chick embryonic sternum mature and express phenotypic characteristics of postmitotic hypertrophic cells. In concert with these maturation-dependent changes, cells release adenine nucleotides into the culture medium. To ascertain if these compounds modulate chondrocyte function, we challenged chondrocytes with nucleotides and measured one determinant of the signal transduction pathway, intracellular Ca2+ concentration ([Ca2+]i). In the presence of micromolar concentrations of ATP, there was a dose-dependent elevation in chondrocyte [Ca2+]i; ADP caused a small but significant rise in the peak [Ca2+]i response. We found that the change in the [Ca2+]i response is linked to retinoid-dependent maturation of chondrocytes. Thus the [Ca2+]i rise was dependent on the RA concentration and treatment time. Immature caudal chondrocytes, cells that were not affected by RA, were used as control cells for this study. When treated with ATP, these cells did not exhibit a [Ca2+]i response. Although the purinergic subtype receptor was not characterized, the observation that cells responded to ATP and ADP but were refractory to AMP and adenosine suggested that P2 purinoc...Continue Reading

References

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Citations

Sep 9, 1998·The American Journal of Physiology·G I GorodeskiF W Abdul-Karim
Jan 6, 2010·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Bo HuoX Edward Guo
Mar 30, 2010·Acta Physiologica·G BurnstockA Verkhratsky
Feb 4, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ning WangAlison Gartland
Jan 3, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yilu ZhouX Lucas Lu
Sep 19, 2019·Biophysics Reviews·Roberta AlfieriFederica Viti
Jan 12, 2011·Cellular and Molecular Life Sciences : CMLS·Geoffrey Burnstock, Henning Ulrich
Nov 25, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·X Lucas LuX Edward Guo
Nov 12, 2016·Journal of Dental Research·M Ishikawa, Y Yamada
Dec 19, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Da JingX Edward Guo
Aug 15, 2013·Purinergic Signalling·Geoffrey BurnstockIsabel R Orriss
May 21, 2015·Purinergic Signalling·Geoffrey Burnstock, Nicholas Dale
Oct 6, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Mengxi LvX Lucas Lu
Mar 1, 2008·Cellular and Molecular Bioengineering·Bo HuoX Edward Guo
Jan 27, 2021·Orthopaedic Surgery·Zheng-Wei Duan, Hua Lu
Mar 15, 2001·Brain Research. Developmental Brain Research·P NikolicP R Thorne
Aug 16, 2021·Purinergic Signalling·Ane Larrañaga-VeraBruce Cronstein

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